CRISPR-U™ Gene Knockout Cell Line
Strategy
KDM7A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KDM7A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KDM7A |
| Gene id | 80853 |
| Organism | Homo sapiens |
| Official full symbol | lysine demethylase 7A |
| Gene type | protein-coding |
| Also known as | JHDM1D |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KDM7A-201 | ENST00000397560.7 | 9220 | 941aa | Protein coding | CCDS43658 | Q6ZMT4-1 | NM_030647.2 | TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| KDM7A-202 | ENST00000472616.1 | 7943 | 404aa | Nonsense mediated decay | - | H9KVD6 | - | CDS 5' incomplete, TSL:1, |
| KDM7A-203 | ENST00000478996.1 | 624 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KDM7A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KDM7A had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: