CRISPR-U™ Gene Knockout Cell Line Strategy

CALM3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CALM3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CALM3
Gene id 808
Organism Homo sapiens
Official full symbol calmodulin 3
Gene type protein-coding
Also known as CALM, CAM1, CAM2, CAMB, CPVT6, CaM, CaMIII, HEL-S-72, LQT16, PHKD, PHKD3
Summary This gene encodes a member of a family of proteins that binds calcium and functions as a enzymatic co-factor. Activity of this protein is important in the regulation of the cell cycle and cytokinesis. Multiple alternatively spliced transcript variants have been observed at this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CALM3-201 ENST00000291295.14 2184 149aa Protein coding CCDS33061 B4DJ51 P0DP25 NM_005184.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CALM3-212 ENST00000599839.5 1355 113aa Protein coding CCDS86783 Q96HY3 - TSL:1, GENCODE basic,
CALM3-208 ENST00000596362.1 1203 149aa Protein coding CCDS86782 B4DJ51 P0DP25 - TSL:2, GENCODE basic, APPRIS P1,
CALM3-202 ENST00000391918.6 702 113aa Protein coding CCDS86783 Q96HY3 - TSL:2, GENCODE basic,
CALM3-211 ENST00000598871.5 561 113aa Protein coding CCDS86783 Q96HY3 - TSL:4, GENCODE basic,
CALM3-206 ENST00000594523.5 540 113aa Protein coding CCDS86783 Q96HY3 - TSL:4, GENCODE basic,
CALM3-209 ENST00000597743.5 577 83aa Protein coding - M0QZ52 - TSL:4, GENCODE basic,
CALM3-213 ENST00000602169.2 880 36aa Nonsense mediated decay - A0A590UJD7 - TSL:2,
CALM3-203 ENST00000477244.5 696 No protein Processed transcript - - - TSL:3,
CALM3-204 ENST00000482455.5 650 No protein Processed transcript - - - TSL:4,
CALM3-207 ENST00000595072.2 2960 No protein Retained intron - - - TSL:2,
CALM3-205 ENST00000486500.1 2536 No protein Retained intron - - - TSL:2,
CALM3-210 ENST00000597868.5 927 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CALM3
This KO Strategy loading
Red Cotton™ Notes Gene CALM3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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