CRISPR-U™ Gene Knockout Cell Line
Strategy
CALM3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CALM3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CALM3 |
| Gene id | 808 |
| Organism | Homo sapiens |
| Official full symbol | calmodulin 3 |
| Gene type | protein-coding |
| Also known as | CALM, CAM1, CAM2, CAMB, CPVT6, CaM, CaMIII, HEL-S-72, LQT16, PHKD, PHKD3 |
| Summary | This gene encodes a member of a family of proteins that binds calcium and functions as a enzymatic co-factor. Activity of this protein is important in the regulation of the cell cycle and cytokinesis. Multiple alternatively spliced transcript variants have been observed at this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CALM3-201 | ENST00000291295.14 | 2184 | 149aa | Protein coding | CCDS33061 | B4DJ51 P0DP25 | NM_005184.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CALM3-212 | ENST00000599839.5 | 1355 | 113aa | Protein coding | CCDS86783 | Q96HY3 | - | TSL:1, GENCODE basic, |
| CALM3-208 | ENST00000596362.1 | 1203 | 149aa | Protein coding | CCDS86782 | B4DJ51 P0DP25 | - | TSL:2, GENCODE basic, APPRIS P1, |
| CALM3-202 | ENST00000391918.6 | 702 | 113aa | Protein coding | CCDS86783 | Q96HY3 | - | TSL:2, GENCODE basic, |
| CALM3-211 | ENST00000598871.5 | 561 | 113aa | Protein coding | CCDS86783 | Q96HY3 | - | TSL:4, GENCODE basic, |
| CALM3-206 | ENST00000594523.5 | 540 | 113aa | Protein coding | CCDS86783 | Q96HY3 | - | TSL:4, GENCODE basic, |
| CALM3-209 | ENST00000597743.5 | 577 | 83aa | Protein coding | - | M0QZ52 | - | TSL:4, GENCODE basic, |
| CALM3-213 | ENST00000602169.2 | 880 | 36aa | Nonsense mediated decay | - | A0A590UJD7 | - | TSL:2, |
| CALM3-203 | ENST00000477244.5 | 696 | No protein | Processed transcript | - | - | - | TSL:3, |
| CALM3-204 | ENST00000482455.5 | 650 | No protein | Processed transcript | - | - | - | TSL:4, |
| CALM3-207 | ENST00000595072.2 | 2960 | No protein | Retained intron | - | - | - | TSL:2, |
| CALM3-205 | ENST00000486500.1 | 2536 | No protein | Retained intron | - | - | - | TSL:2, |
| CALM3-210 | ENST00000597868.5 | 927 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CALM3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CALM3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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