CRISPR-U™ Gene Knockout Cell Line Strategy

UBXN6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human UBXN6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol UBXN6
Gene id 80700
Organism Homo sapiens
Official full symbol UBX domain protein 6
Gene type protein-coding
Also known as UBXD1, UBXDC2
Genomic regions Chromosome 19
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
UBXN6-201 ENST00000301281.11 1915 441aa Protein coding CCDS12129 Q9BZV1-1 NM_025241.3 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
UBXN6-202 ENST00000394765.7 1809 388aa Protein coding CCDS54201 Q9BZV1-2 - TSL:1, GENCODE basic, APPRIS ALT1,
UBXN6-207 ENST00000591919.5 1613 371aa Protein coding - K7EP32 - CDS 5' incomplete, TSL:5,
UBXN6-209 ENST00000592515.1 403 129aa Protein coding - K7EJ02 - CDS 3' incomplete, TSL:5,
UBXN6-206 ENST00000590466.2 803 67aa Nonsense mediated decay - K7ELN1 - CDS 5' incomplete, TSL:3,
UBXN6-205 ENST00000588238.1 4598 No protein Retained intron - - - TSL:NA,
UBXN6-203 ENST00000587009.5 1967 No protein Retained intron - - - TSL:2,
UBXN6-204 ENST00000587324.1 712 No protein Retained intron - - - TSL:3,
UBXN6-210 ENST00000593024.1 629 No protein Retained intron - - - TSL:2,
UBXN6-208 ENST00000592358.1 626 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene UBXN6
This KO Strategy loading
Red Cotton™ Notes Gene UBXN6 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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