CRISPR-U™ Gene Knockout Cell Line
Strategy
PUS1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PUS1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PUS1 |
| Gene id | 80324 |
| Organism | Homo sapiens |
| Official full symbol | pseudouridine synthase 1 |
| Gene type | protein-coding |
| Also known as | MLASA1 |
| Summary | This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PUS1-202 | ENST00000376649.8 | 4041 | 427aa | Protein coding | CCDS9275 | Q9Y606-1 | NM_025215.6 | TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| PUS1-203 | ENST00000443358.6 | 1664 | 399aa | Protein coding | CCDS31928 | Q9Y606-2 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PUS1-208 | ENST00000542167.2 | 2151 | 374aa | Protein coding | - | F5H1S9 | - | TSL:5, GENCODE basic, |
| PUS1-201 | ENST00000322060.9 | 1206 | 384aa | Protein coding | - | G8JLB3 | - | CDS 3' incomplete, TSL:5, |
| PUS1-205 | ENST00000535067.5 | 838 | 134aa | Protein coding | - | F5H168 | - | TSL:3, GENCODE basic, |
| PUS1-210 | ENST00000544213.5 | 687 | 184aa | Protein coding | - | F5H1B2 | - | CDS 3' incomplete, TSL:5, |
| PUS1-206 | ENST00000537484.1 | 642 | 200aa | Protein coding | - | F5GXL3 | - | CDS 3' incomplete, TSL:3, |
| PUS1-207 | ENST00000538037.5 | 631 | 153aa | Protein coding | - | F5GY32 | - | CDS 3' incomplete, TSL:5, |
| PUS1-204 | ENST00000456665.6 | 577 | 156aa | Protein coding | - | F8W9U5 | - | CDS 3' incomplete, TSL:5, |
| PUS1-209 | ENST00000543754.1 | 1378 | No protein | Retained intron | - | - | - | TSL:2, |
| PUS1-211 | ENST00000544662.1 | 836 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PUS1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PUS1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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