CRISPR-U™ Gene Knockout Cell Line Strategy

PUS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PUS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PUS1
Gene id 80324
Organism Homo sapiens
Official full symbol pseudouridine synthase 1
Gene type protein-coding
Also known as MLASA1
Summary This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 12
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PUS1-202 ENST00000376649.8 4041 427aa Protein coding CCDS9275 Q9Y606-1 NM_025215.6 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
PUS1-203 ENST00000443358.6 1664 399aa Protein coding CCDS31928 Q9Y606-2 - TSL:1, GENCODE basic, APPRIS P4,
PUS1-208 ENST00000542167.2 2151 374aa Protein coding - F5H1S9 - TSL:5, GENCODE basic,
PUS1-201 ENST00000322060.9 1206 384aa Protein coding - G8JLB3 - CDS 3' incomplete, TSL:5,
PUS1-205 ENST00000535067.5 838 134aa Protein coding - F5H168 - TSL:3, GENCODE basic,
PUS1-210 ENST00000544213.5 687 184aa Protein coding - F5H1B2 - CDS 3' incomplete, TSL:5,
PUS1-206 ENST00000537484.1 642 200aa Protein coding - F5GXL3 - CDS 3' incomplete, TSL:3,
PUS1-207 ENST00000538037.5 631 153aa Protein coding - F5GY32 - CDS 3' incomplete, TSL:5,
PUS1-204 ENST00000456665.6 577 156aa Protein coding - F8W9U5 - CDS 3' incomplete, TSL:5,
PUS1-209 ENST00000543754.1 1378 No protein Retained intron - - - TSL:2,
PUS1-211 ENST00000544662.1 836 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PUS1
This KO Strategy loading
Red Cotton™ Notes Gene PUS1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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