CRISPR-U™ Gene Knockout Cell Line Strategy

PDGFD Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PDGFD Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PDGFD
Gene id 80310
Organism Homo sapiens
Official full symbol platelet derived growth factor D
Gene type protein-coding
Also known as IEGF, MSTP036, SCDGF-B, SCDGFB
Summary The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PDGFD-201 ENST00000302251.9 4052 364aa Protein coding CCDS8326 Q9GZP0-2 - TSL:1, GENCODE basic,
PDGFD-202 ENST00000393158.7 3838 370aa Protein coding CCDS41703 Q9GZP0-1 NM_025208.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PDGFD-203 ENST00000529268.1 620 206aa Protein coding - H0YD37 - CDS 5' and 3' incomplete, TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PDGFD
This KO Strategy loading
Red Cotton™ Notes Gene PDGFD had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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