CRISPR-U™ Gene Knockout Cell Line
Strategy
PDGFD Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PDGFD Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PDGFD |
| Gene id | 80310 |
| Organism | Homo sapiens |
| Official full symbol | platelet derived growth factor D |
| Gene type | protein-coding |
| Also known as | IEGF, MSTP036, SCDGF-B, SCDGFB |
| Summary | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PDGFD-201 | ENST00000302251.9 | 4052 | 364aa | Protein coding | CCDS8326 | Q9GZP0-2 | - | TSL:1, GENCODE basic, |
| PDGFD-202 | ENST00000393158.7 | 3838 | 370aa | Protein coding | CCDS41703 | Q9GZP0-1 | NM_025208.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PDGFD-203 | ENST00000529268.1 | 620 | 206aa | Protein coding | - | H0YD37 | - | CDS 5' and 3' incomplete, TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PDGFD |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PDGFD had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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