CRISPR-U™ Gene Knockout Cell Line Strategy

CCDC6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CCDC6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CCDC6
Gene id 8030
Organism Homo sapiens
Official full symbol coiled-coil domain containing 6
Gene type protein-coding
Also known as D10S170, H4, PTC, TPC, TST1
Summary This gene encodes a coiled-coil domain-containing protein. The encoded protein is ubiquitously expressed and may function as a tumor suppressor. A chromosomal rearrangement resulting in the expression of a fusion gene containing a portion of this gene and the intracellular kinase-encoding domain of the ret proto-oncogene is the cause of thyroid papillary carcinoma.
Genomic regions Chromosome 10
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CCDC6-201 ENST00000263102.7 5727 474aa Protein coding CCDS7257 Q16204 NM_005436.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CCDC6-202 ENST00000491922.1 2642 No protein Retained intron - - - TSL:2,
CCDC6-203 ENST00000518638.1 729 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CCDC6
This KO Strategy loading
Red Cotton™ Notes Gene CCDC6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
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