CRISPR-U™ Gene Knockout Cell Line
Strategy
CCDC6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CCDC6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CCDC6 |
| Gene id | 8030 |
| Organism | Homo sapiens |
| Official full symbol | coiled-coil domain containing 6 |
| Gene type | protein-coding |
| Also known as | D10S170, H4, PTC, TPC, TST1 |
| Summary | This gene encodes a coiled-coil domain-containing protein. The encoded protein is ubiquitously expressed and may function as a tumor suppressor. A chromosomal rearrangement resulting in the expression of a fusion gene containing a portion of this gene and the intracellular kinase-encoding domain of the ret proto-oncogene is the cause of thyroid papillary carcinoma. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CCDC6-201 | ENST00000263102.7 | 5727 | 474aa | Protein coding | CCDS7257 | Q16204 | NM_005436.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CCDC6-202 | ENST00000491922.1 | 2642 | No protein | Retained intron | - | - | - | TSL:2, |
| CCDC6-203 | ENST00000518638.1 | 729 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CCDC6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CCDC6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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