CRISPR-U™ Gene Knockout Cell Line Strategy

HSD3B7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HSD3B7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HSD3B7
Gene id 80270
Organism Homo sapiens
Official full symbol hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
Gene type protein-coding
Also known as CBAS1, PFIC4, SDR11E3
Summary This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 16
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HSD3B7-201 ENST00000262520.10 2200 196aa Protein coding CCDS45466 Q9H2F3-2 - TSL:2, GENCODE basic,
HSD3B7-202 ENST00000297679.10 2171 369aa Protein coding CCDS10698 Q9H2F3-1 NM_025193.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HSD3B7-203 ENST00000562932.5 746 219aa Protein coding - I3L2Q9 - CDS 3' incomplete, TSL:5,
HSD3B7-204 ENST00000574447.1 746 176aa Protein coding - I3L2H6 - CDS 3' incomplete, TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HSD3B7
This KO Strategy loading
Red Cotton™ Notes Gene HSD3B7 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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