CRISPR-U™ Gene Knockout Cell Line
Strategy
HSD3B7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HSD3B7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HSD3B7 |
| Gene id | 80270 |
| Organism | Homo sapiens |
| Official full symbol | hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 |
| Gene type | protein-coding |
| Also known as | CBAS1, PFIC4, SDR11E3 |
| Summary | This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HSD3B7-201 | ENST00000262520.10 | 2200 | 196aa | Protein coding | CCDS45466 | Q9H2F3-2 | - | TSL:2, GENCODE basic, |
| HSD3B7-202 | ENST00000297679.10 | 2171 | 369aa | Protein coding | CCDS10698 | Q9H2F3-1 | NM_025193.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HSD3B7-203 | ENST00000562932.5 | 746 | 219aa | Protein coding | - | I3L2Q9 | - | CDS 3' incomplete, TSL:5, |
| HSD3B7-204 | ENST00000574447.1 | 746 | 176aa | Protein coding | - | I3L2H6 | - | CDS 3' incomplete, TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HSD3B7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HSD3B7 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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