CRISPR-U™ Gene Knockout Cell Line
Strategy
BRD3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BRD3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BRD3 |
| Gene id | 8019 |
| Organism | Homo sapiens |
| Official full symbol | bromodomain containing 3 |
| Gene type | protein-coding |
| Also known as | ORFX, RING3L |
| Summary | This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BRD3-201 | ENST00000303407.12 | 5661 | 726aa | Protein coding | CCDS6980 | Q15059-1 | NM_007371.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BRD3-202 | ENST00000371834.6 | 2529 | 556aa | Protein coding | - | Q15059-2 | - | TSL:1, GENCODE basic, |
| BRD3-204 | ENST00000433041.1 | 599 | 20aa | Protein coding | - | A9J575 | - | CDS 3' incomplete, TSL:3, |
| BRD3-203 | ENST00000371842.2 | 574 | 74aa | Protein coding | - | A9J571 | - | CDS 3' incomplete, TSL:5, |
| BRD3-205 | ENST00000473349.1 | 761 | No protein | Processed transcript | - | - | - | TSL:3, |
| BRD3-206 | ENST00000494743.1 | 507 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BRD3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BRD3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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