CRISPR-U™ Gene Knockout Cell Line Strategy

BRD3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BRD3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BRD3
Gene id 8019
Organism Homo sapiens
Official full symbol bromodomain containing 3
Gene type protein-coding
Also known as ORFX, RING3L
Summary This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.
Genomic regions Chromosome 9
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BRD3-201 ENST00000303407.12 5661 726aa Protein coding CCDS6980 Q15059-1 NM_007371.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BRD3-202 ENST00000371834.6 2529 556aa Protein coding - Q15059-2 - TSL:1, GENCODE basic,
BRD3-204 ENST00000433041.1 599 20aa Protein coding - A9J575 - CDS 3' incomplete, TSL:3,
BRD3-203 ENST00000371842.2 574 74aa Protein coding - A9J571 - CDS 3' incomplete, TSL:5,
BRD3-205 ENST00000473349.1 761 No protein Processed transcript - - - TSL:3,
BRD3-206 ENST00000494743.1 507 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BRD3
This KO Strategy loading
Red Cotton™ Notes Gene BRD3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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