CRISPR-U™ Gene Knockout Cell Line
Strategy
SIKE1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SIKE1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SIKE1 |
| Gene id | 80143 |
| Organism | Homo sapiens |
| Official full symbol | suppressor of IKBKE 1 |
| Gene type | protein-coding |
| Also known as | SIKE |
| Summary | SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 ). |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SIKE1-203 | ENST00000369528.9 | 5506 | 211aa | Protein coding | CCDS41371 | Q9BRV8-2 | - | TSL:1, GENCODE basic, |
| SIKE1-201 | ENST00000060969.6 | 5494 | 207aa | Protein coding | CCDS878 | Q9BRV8-1 | NM_025073.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SIKE1-209 | ENST00000506320.1 | 505 | No protein | Processed transcript | - | - | - | TSL:2, |
| SIKE1-208 | ENST00000475335.5 | 470 | No protein | Processed transcript | - | - | - | TSL:2, |
| SIKE1-202 | ENST00000369524.5 | 455 | No protein | Processed transcript | - | - | - | TSL:3, |
| SIKE1-205 | ENST00000466657.1 | 415 | No protein | Processed transcript | - | - | - | TSL:3, |
| SIKE1-207 | ENST00000474562.1 | 412 | No protein | Processed transcript | - | - | - | TSL:2, |
| SIKE1-204 | ENST00000462123.5 | 394 | No protein | Processed transcript | - | - | - | TSL:2, |
| SIKE1-210 | ENST00000510745.5 | 349 | No protein | Processed transcript | - | - | - | TSL:2, |
| SIKE1-206 | ENST00000471969.1 | 469 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SIKE1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SIKE1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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