CRISPR-U™ Gene Knockout Cell Line Strategy

SIKE1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SIKE1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SIKE1
Gene id 80143
Organism Homo sapiens
Official full symbol suppressor of IKBKE 1
Gene type protein-coding
Also known as SIKE
Summary SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 ).
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SIKE1-203 ENST00000369528.9 5506 211aa Protein coding CCDS41371 Q9BRV8-2 - TSL:1, GENCODE basic,
SIKE1-201 ENST00000060969.6 5494 207aa Protein coding CCDS878 Q9BRV8-1 NM_025073.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SIKE1-209 ENST00000506320.1 505 No protein Processed transcript - - - TSL:2,
SIKE1-208 ENST00000475335.5 470 No protein Processed transcript - - - TSL:2,
SIKE1-202 ENST00000369524.5 455 No protein Processed transcript - - - TSL:3,
SIKE1-205 ENST00000466657.1 415 No protein Processed transcript - - - TSL:3,
SIKE1-207 ENST00000474562.1 412 No protein Processed transcript - - - TSL:2,
SIKE1-204 ENST00000462123.5 394 No protein Processed transcript - - - TSL:2,
SIKE1-210 ENST00000510745.5 349 No protein Processed transcript - - - TSL:2,
SIKE1-206 ENST00000471969.1 469 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SIKE1
This KO Strategy loading
Red Cotton™ Notes Gene SIKE1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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