CRISPR-U™ Gene Knockout Cell Line
Strategy
CALD1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CALD1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CALD1 |
| Gene id | 800 |
| Organism | Homo sapiens |
| Official full symbol | caldesmon 1 |
| Gene type | protein-coding |
| Also known as | CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22 |
| Summary | This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CALD1-201 | ENST00000361675.7 | 5011 | 793aa | Protein coding | CCDS5835 | Q05682-1 | NM_033138.4 | TSL:1, GENCODE basic, MANE Select v0.92, |
| CALD1-203 | ENST00000393118.6 | 4281 | 558aa | Protein coding | CCDS47716 | Q05682-3 | - | TSL:1, GENCODE basic, |
| CALD1-202 | ENST00000361901.6 | 4114 | 538aa | Protein coding | CCDS5834 | Q05682-4 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CALD1-205 | ENST00000422748.5 | 2665 | 563aa | Protein coding | CCDS5836 | Q05682-6 | - | TSL:2, GENCODE basic, |
| CALD1-206 | ENST00000424922.5 | 2124 | 532aa | Protein coding | CCDS47717 | Q05682-5 | - | TSL:1, GENCODE basic, |
| CALD1-204 | ENST00000417172.5 | 1915 | 538aa | Protein coding | CCDS5834 | Q05682-4 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CALD1-222 | ENST00000495522.1 | 2199 | 557aa | Protein coding | - | E7EX44 | - | TSL:5, GENCODE basic, |
| CALD1-209 | ENST00000436461.6 | 1614 | 459aa | Protein coding | - | C9J813 | - | CDS 3' incomplete, TSL:5, |
| CALD1-214 | ENST00000466704.1 | 903 | 205aa | Protein coding | - | - | - | CDS 5' incomplete, TSL:3, |
| CALD1-212 | ENST00000454108.5 | 558 | 72aa | Protein coding | - | C9JE79 | - | CDS 3' incomplete, TSL:5, |
| CALD1-211 | ENST00000445569.6 | 525 | 132aa | Protein coding | - | C9JEK3 | - | CDS 3' incomplete, TSL:5, |
| CALD1-208 | ENST00000435928.1 | 458 | 72aa | Protein coding | - | C9JE79 | - | CDS 3' incomplete, TSL:5, |
| CALD1-210 | ENST00000443197.5 | 4173 | 536aa | Nonsense mediated decay | - | E9PGZ1 | - | TSL:5, |
| CALD1-207 | ENST00000430085.5 | 1938 | 85aa | Nonsense mediated decay | - | F8WE61 | - | TSL:2, |
| CALD1-223 | ENST00000496024.1 | 557 | No protein | Processed transcript | - | - | - | TSL:4, |
| CALD1-221 | ENST00000489019.6 | 422 | No protein | Processed transcript | - | - | - | TSL:5, |
| CALD1-218 | ENST00000478075.1 | 193 | No protein | Processed transcript | - | - | - | TSL:3, |
| CALD1-213 | ENST00000462181.1 | 188 | No protein | Processed transcript | - | - | - | TSL:5, |
| CALD1-220 | ENST00000482470.5 | 3941 | No protein | Retained intron | - | - | - | TSL:2, |
| CALD1-217 | ENST00000475772.5 | 753 | No protein | Retained intron | - | - | - | TSL:2, |
| CALD1-215 | ENST00000472096.1 | 651 | No protein | Retained intron | - | - | - | TSL:2, |
| CALD1-219 | ENST00000480638.1 | 560 | No protein | Retained intron | - | - | - | TSL:2, |
| CALD1-224 | ENST00000498254.5 | 443 | No protein | Retained intron | - | - | - | TSL:3, |
| CALD1-216 | ENST00000473714.1 | 230 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CALD1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CALD1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: