CRISPR-U™ Gene Knockout Cell Line Strategy

CALD1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CALD1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CALD1
Gene id 800
Organism Homo sapiens
Official full symbol caldesmon 1
Gene type protein-coding
Also known as CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22
Summary This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Genomic regions Chromosome 7
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CALD1-201 ENST00000361675.7 5011 793aa Protein coding CCDS5835 Q05682-1 NM_033138.4 TSL:1, GENCODE basic, MANE Select v0.92,
CALD1-203 ENST00000393118.6 4281 558aa Protein coding CCDS47716 Q05682-3 - TSL:1, GENCODE basic,
CALD1-202 ENST00000361901.6 4114 538aa Protein coding CCDS5834 Q05682-4 - TSL:1, GENCODE basic, APPRIS P1,
CALD1-205 ENST00000422748.5 2665 563aa Protein coding CCDS5836 Q05682-6 - TSL:2, GENCODE basic,
CALD1-206 ENST00000424922.5 2124 532aa Protein coding CCDS47717 Q05682-5 - TSL:1, GENCODE basic,
CALD1-204 ENST00000417172.5 1915 538aa Protein coding CCDS5834 Q05682-4 - TSL:5, GENCODE basic, APPRIS P1,
CALD1-222 ENST00000495522.1 2199 557aa Protein coding - E7EX44 - TSL:5, GENCODE basic,
CALD1-209 ENST00000436461.6 1614 459aa Protein coding - C9J813 - CDS 3' incomplete, TSL:5,
CALD1-214 ENST00000466704.1 903 205aa Protein coding - - - CDS 5' incomplete, TSL:3,
CALD1-212 ENST00000454108.5 558 72aa Protein coding - C9JE79 - CDS 3' incomplete, TSL:5,
CALD1-211 ENST00000445569.6 525 132aa Protein coding - C9JEK3 - CDS 3' incomplete, TSL:5,
CALD1-208 ENST00000435928.1 458 72aa Protein coding - C9JE79 - CDS 3' incomplete, TSL:5,
CALD1-210 ENST00000443197.5 4173 536aa Nonsense mediated decay - E9PGZ1 - TSL:5,
CALD1-207 ENST00000430085.5 1938 85aa Nonsense mediated decay - F8WE61 - TSL:2,
CALD1-223 ENST00000496024.1 557 No protein Processed transcript - - - TSL:4,
CALD1-221 ENST00000489019.6 422 No protein Processed transcript - - - TSL:5,
CALD1-218 ENST00000478075.1 193 No protein Processed transcript - - - TSL:3,
CALD1-213 ENST00000462181.1 188 No protein Processed transcript - - - TSL:5,
CALD1-220 ENST00000482470.5 3941 No protein Retained intron - - - TSL:2,
CALD1-217 ENST00000475772.5 753 No protein Retained intron - - - TSL:2,
CALD1-215 ENST00000472096.1 651 No protein Retained intron - - - TSL:2,
CALD1-219 ENST00000480638.1 560 No protein Retained intron - - - TSL:2,
CALD1-224 ENST00000498254.5 443 No protein Retained intron - - - TSL:3,
CALD1-216 ENST00000473714.1 230 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CALD1
This KO Strategy loading
Red Cotton™ Notes Gene CALD1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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