CRISPR-U™ Gene Knockout Cell Line Strategy

NHEJ1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NHEJ1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NHEJ1
Gene id 79840
Organism Homo sapiens
Official full symbol non-homologous end joining factor 1
Gene type protein-coding
Also known as XLF
Summary Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
Genomic regions Chromosome 2
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NHEJ1-201 ENST00000356853.10 8020 299aa Protein coding CCDS2432 Q9H9Q4-1 NM_024782.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
NHEJ1-202 ENST00000409720.5 1954 316aa Protein coding - Q9H9Q4-2 - TSL:5, GENCODE basic, APPRIS ALT2,
NHEJ1-204 ENST00000426304.5 753 224aa Protein coding - H7C0G7 - CDS 5' incomplete, TSL:3,
NHEJ1-206 ENST00000457600.2 734 229aa Protein coding - C9JWV4 - CDS 3' incomplete, TSL:3,
NHEJ1-203 ENST00000418099.5 2113 232aa Nonsense mediated decay - G5E9Q8 - TSL:5,
NHEJ1-205 ENST00000450447.1 547 50aa Nonsense mediated decay - F8WEB8 - TSL:4,
NHEJ1-208 ENST00000483627.1 587 No protein Processed transcript - - - TSL:3,
NHEJ1-209 ENST00000491159.5 572 No protein Processed transcript - - - TSL:4,
NHEJ1-207 ENST00000481764.1 555 No protein Processed transcript - - - TSL:4,
NHEJ1-210 ENST00000494211.5 555 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NHEJ1
This KO Strategy loading
Red Cotton™ Notes Gene NHEJ1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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