CRISPR-U™ Gene Knockout Cell Line
Strategy
NHEJ1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NHEJ1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NHEJ1 |
| Gene id | 79840 |
| Organism | Homo sapiens |
| Official full symbol | non-homologous end joining factor 1 |
| Gene type | protein-coding |
| Also known as | XLF |
| Summary | Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NHEJ1-201 | ENST00000356853.10 | 8020 | 299aa | Protein coding | CCDS2432 | Q9H9Q4-1 | NM_024782.3 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| NHEJ1-202 | ENST00000409720.5 | 1954 | 316aa | Protein coding | - | Q9H9Q4-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| NHEJ1-204 | ENST00000426304.5 | 753 | 224aa | Protein coding | - | H7C0G7 | - | CDS 5' incomplete, TSL:3, |
| NHEJ1-206 | ENST00000457600.2 | 734 | 229aa | Protein coding | - | C9JWV4 | - | CDS 3' incomplete, TSL:3, |
| NHEJ1-203 | ENST00000418099.5 | 2113 | 232aa | Nonsense mediated decay | - | G5E9Q8 | - | TSL:5, |
| NHEJ1-205 | ENST00000450447.1 | 547 | 50aa | Nonsense mediated decay | - | F8WEB8 | - | TSL:4, |
| NHEJ1-208 | ENST00000483627.1 | 587 | No protein | Processed transcript | - | - | - | TSL:3, |
| NHEJ1-209 | ENST00000491159.5 | 572 | No protein | Processed transcript | - | - | - | TSL:4, |
| NHEJ1-207 | ENST00000481764.1 | 555 | No protein | Processed transcript | - | - | - | TSL:4, |
| NHEJ1-210 | ENST00000494211.5 | 555 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NHEJ1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NHEJ1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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