CRISPR-U™ Gene Knockout Cell Line Strategy

IPO4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IPO4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IPO4
Gene id 79711
Organism Homo sapiens
Official full symbol importin 4
Gene type protein-coding
Also known as Imp4
Genomic regions Chromosome 14
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IPO4-201 ENST00000354464.11 3498 1081aa Protein coding CCDS9616 Q8TEX9-1 NM_024658.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IPO4-220 ENST00000561462.5 1864 547aa Protein coding - H0YMR4 - CDS 5' incomplete, TSL:2,
IPO4-205 ENST00000558233.1 1015 312aa Protein coding - H0YN07 - CDS 5' incomplete, TSL:5,
IPO4-221 ENST00000625289.1 297 98aa Protein coding - H0YLV0 - TSL:5, GENCODE basic,
IPO4-207 ENST00000558780.5 3540 101aa Nonsense mediated decay - H0YL92 - TSL:2,
IPO4-211 ENST00000560155.5 3485 911aa Nonsense mediated decay - H0YN14 - TSL:1,
IPO4-203 ENST00000558046.5 3440 154aa Nonsense mediated decay - H0YKG5 - TSL:2,
IPO4-208 ENST00000559253.1 579 98aa Nonsense mediated decay - H0YLV0 - TSL:4,
IPO4-216 ENST00000561034.1 535 48aa Nonsense mediated decay - H0YK93 - TSL:4,
IPO4-217 ENST00000561090.5 3632 No protein Retained intron - - - TSL:2,
IPO4-214 ENST00000560798.5 3581 No protein Retained intron - - - TSL:2,
IPO4-209 ENST00000559588.5 3537 No protein Retained intron - - - TSL:1,
IPO4-218 ENST00000561199.5 3249 No protein Retained intron - - - TSL:2,
IPO4-215 ENST00000560935.1 701 No protein Retained intron - - - TSL:3,
IPO4-219 ENST00000561379.1 624 No protein Retained intron - - - TSL:4,
IPO4-212 ENST00000560222.1 573 No protein Retained intron - - - TSL:3,
IPO4-213 ENST00000560315.5 558 No protein Retained intron - - - TSL:2,
IPO4-204 ENST00000558193.5 555 No protein Retained intron - - - TSL:4,
IPO4-206 ENST00000558718.5 554 No protein Retained intron - - - TSL:2,
IPO4-202 ENST00000557996.1 530 No protein Retained intron - - - TSL:4,
IPO4-210 ENST00000559635.1 492 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IPO4
This KO Strategy loading
Red Cotton™ Notes Gene IPO4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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