CRISPR-U™ Gene Knockout Cell Line
Strategy
IPO4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IPO4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IPO4 |
| Gene id | 79711 |
| Organism | Homo sapiens |
| Official full symbol | importin 4 |
| Gene type | protein-coding |
| Also known as | Imp4 |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IPO4-201 | ENST00000354464.11 | 3498 | 1081aa | Protein coding | CCDS9616 | Q8TEX9-1 | NM_024658.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IPO4-220 | ENST00000561462.5 | 1864 | 547aa | Protein coding | - | H0YMR4 | - | CDS 5' incomplete, TSL:2, |
| IPO4-205 | ENST00000558233.1 | 1015 | 312aa | Protein coding | - | H0YN07 | - | CDS 5' incomplete, TSL:5, |
| IPO4-221 | ENST00000625289.1 | 297 | 98aa | Protein coding | - | H0YLV0 | - | TSL:5, GENCODE basic, |
| IPO4-207 | ENST00000558780.5 | 3540 | 101aa | Nonsense mediated decay | - | H0YL92 | - | TSL:2, |
| IPO4-211 | ENST00000560155.5 | 3485 | 911aa | Nonsense mediated decay | - | H0YN14 | - | TSL:1, |
| IPO4-203 | ENST00000558046.5 | 3440 | 154aa | Nonsense mediated decay | - | H0YKG5 | - | TSL:2, |
| IPO4-208 | ENST00000559253.1 | 579 | 98aa | Nonsense mediated decay | - | H0YLV0 | - | TSL:4, |
| IPO4-216 | ENST00000561034.1 | 535 | 48aa | Nonsense mediated decay | - | H0YK93 | - | TSL:4, |
| IPO4-217 | ENST00000561090.5 | 3632 | No protein | Retained intron | - | - | - | TSL:2, |
| IPO4-214 | ENST00000560798.5 | 3581 | No protein | Retained intron | - | - | - | TSL:2, |
| IPO4-209 | ENST00000559588.5 | 3537 | No protein | Retained intron | - | - | - | TSL:1, |
| IPO4-218 | ENST00000561199.5 | 3249 | No protein | Retained intron | - | - | - | TSL:2, |
| IPO4-215 | ENST00000560935.1 | 701 | No protein | Retained intron | - | - | - | TSL:3, |
| IPO4-219 | ENST00000561379.1 | 624 | No protein | Retained intron | - | - | - | TSL:4, |
| IPO4-212 | ENST00000560222.1 | 573 | No protein | Retained intron | - | - | - | TSL:3, |
| IPO4-213 | ENST00000560315.5 | 558 | No protein | Retained intron | - | - | - | TSL:2, |
| IPO4-204 | ENST00000558193.5 | 555 | No protein | Retained intron | - | - | - | TSL:4, |
| IPO4-206 | ENST00000558718.5 | 554 | No protein | Retained intron | - | - | - | TSL:2, |
| IPO4-202 | ENST00000557996.1 | 530 | No protein | Retained intron | - | - | - | TSL:4, |
| IPO4-210 | ENST00000559635.1 | 492 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IPO4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IPO4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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