CRISPR-U™ Gene Knockout Cell Line
Strategy
LRRK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LRRK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LRRK1 |
| Gene id | 79705 |
| Organism | Homo sapiens |
| Official full symbol | leucine rich repeat kinase 1 |
| Gene type | protein-coding |
| Also known as | RIPK6, Roco1 |
| Summary | This gene encodes a multi-domain protein that is a leucine-rich repeat kinase and a GDP/GTP binding protein. The encoded protein is thought to play a role in the regulation of bone mass. Mice lacking a similar gene showed severe osteopetrosis, increased bone mineralization and decreased bone resorption. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LRRK1-201 | ENST00000388948.8 | 15674 | 2015aa | Protein coding | CCDS42086 | Q38SD2-1 | NM_024652.6 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LRRK1-208 | ENST00000532029.6 | 1241 | 264aa | Protein coding | - | Q38SD2-2 | - | TSL:1, GENCODE basic, |
| LRRK1-210 | ENST00000534045.5 | 569 | 32aa | Protein coding | - | E9PLF8 | - | CDS 3' incomplete, TSL:3, |
| LRRK1-205 | ENST00000526457.3 | 11632 | 660aa | Nonsense mediated decay | - | H0YEW1 | - | CDS 5' incomplete, TSL:1, |
| LRRK1-202 | ENST00000525284.5 | 6494 | 689aa | Nonsense mediated decay | - | E9PMK9 | - | TSL:1, |
| LRRK1-207 | ENST00000531270.5 | 6015 | 650aa | Nonsense mediated decay | - | E9PK39 | - | TSL:1, |
| LRRK1-209 | ENST00000532145.1 | 2790 | No protein | Processed transcript | - | - | - | TSL:2, |
| LRRK1-203 | ENST00000525395.2 | 737 | No protein | Processed transcript | - | - | - | TSL:5, |
| LRRK1-206 | ENST00000527698.1 | 679 | No protein | Retained intron | - | - | - | TSL:2, |
| LRRK1-204 | ENST00000525617.2 | 614 | No protein | Retained intron | - | - | - | TSL:5, |
| LRRK1-211 | ENST00000538064.1 | 493 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LRRK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LRRK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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