CRISPR-U™ Gene Knockout Cell Line Strategy

LRRK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LRRK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LRRK1
Gene id 79705
Organism Homo sapiens
Official full symbol leucine rich repeat kinase 1
Gene type protein-coding
Also known as RIPK6, Roco1
Summary This gene encodes a multi-domain protein that is a leucine-rich repeat kinase and a GDP/GTP binding protein. The encoded protein is thought to play a role in the regulation of bone mass. Mice lacking a similar gene showed severe osteopetrosis, increased bone mineralization and decreased bone resorption.
Genomic regions Chromosome 15
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LRRK1-201 ENST00000388948.8 15674 2015aa Protein coding CCDS42086 Q38SD2-1 NM_024652.6 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
LRRK1-208 ENST00000532029.6 1241 264aa Protein coding - Q38SD2-2 - TSL:1, GENCODE basic,
LRRK1-210 ENST00000534045.5 569 32aa Protein coding - E9PLF8 - CDS 3' incomplete, TSL:3,
LRRK1-205 ENST00000526457.3 11632 660aa Nonsense mediated decay - H0YEW1 - CDS 5' incomplete, TSL:1,
LRRK1-202 ENST00000525284.5 6494 689aa Nonsense mediated decay - E9PMK9 - TSL:1,
LRRK1-207 ENST00000531270.5 6015 650aa Nonsense mediated decay - E9PK39 - TSL:1,
LRRK1-209 ENST00000532145.1 2790 No protein Processed transcript - - - TSL:2,
LRRK1-203 ENST00000525395.2 737 No protein Processed transcript - - - TSL:5,
LRRK1-206 ENST00000527698.1 679 No protein Retained intron - - - TSL:2,
LRRK1-204 ENST00000525617.2 614 No protein Retained intron - - - TSL:5,
LRRK1-211 ENST00000538064.1 493 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LRRK1
This KO Strategy loading
Red Cotton™ Notes Gene LRRK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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