CRISPR-U™ Gene Knockout Cell Line
Strategy
DHX58 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DHX58 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DHX58 |
| Gene id | 79132 |
| Organism | Homo sapiens |
| Official full symbol | DExH-box helicase 58 |
| Gene type | protein-coding |
| Also known as | D11LGP2, D11lgp2e, LGP2, RLR-3 |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DHX58-201 | ENST00000251642.8 | 2591 | 678aa | Protein coding | CCDS11416 | A0A024R1Y5 Q96C10 | NM_024119.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DHX58-202 | ENST00000413196.6 | 1283 | 302aa | Protein coding | - | C9JG98 | - | CDS 3' incomplete, TSL:5, |
| DHX58-203 | ENST00000430773.1 | 730 | 156aa | Protein coding | - | C9JE76 | - | CDS 3' incomplete, TSL:3, |
| DHX58-207 | ENST00000591220.5 | 540 | 99aa | Protein coding | - | K7EMZ5 | - | CDS 3' incomplete, TSL:4, |
| DHX58-205 | ENST00000589979.1 | 782 | 51aa | Nonsense mediated decay | - | K7EPP0 | - | CDS 5' incomplete, TSL:3, |
| DHX58-204 | ENST00000586522.5 | 2331 | No protein | Retained intron | - | - | - | TSL:2, |
| DHX58-208 | ENST00000592024.1 | 941 | No protein | Retained intron | - | - | - | TSL:2, |
| DHX58-206 | ENST00000590637.1 | 748 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DHX58 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DHX58 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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