CRISPR-U™ Gene Knockout Cell Line Strategy

DHX58 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DHX58 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DHX58
Gene id 79132
Organism Homo sapiens
Official full symbol DExH-box helicase 58
Gene type protein-coding
Also known as D11LGP2, D11lgp2e, LGP2, RLR-3
Genomic regions Chromosome 17
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DHX58-201 ENST00000251642.8 2591 678aa Protein coding CCDS11416 A0A024R1Y5 Q96C10 NM_024119.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DHX58-202 ENST00000413196.6 1283 302aa Protein coding - C9JG98 - CDS 3' incomplete, TSL:5,
DHX58-203 ENST00000430773.1 730 156aa Protein coding - C9JE76 - CDS 3' incomplete, TSL:3,
DHX58-207 ENST00000591220.5 540 99aa Protein coding - K7EMZ5 - CDS 3' incomplete, TSL:4,
DHX58-205 ENST00000589979.1 782 51aa Nonsense mediated decay - K7EPP0 - CDS 5' incomplete, TSL:3,
DHX58-204 ENST00000586522.5 2331 No protein Retained intron - - - TSL:2,
DHX58-208 ENST00000592024.1 941 No protein Retained intron - - - TSL:2,
DHX58-206 ENST00000590637.1 748 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DHX58
This KO Strategy loading
Red Cotton™ Notes Gene DHX58 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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