CRISPR-U™ Gene Knockout Cell Line Strategy

CACNB3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CACNB3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CACNB3
Gene id 784
Organism Homo sapiens
Official full symbol calcium voltage-gated channel auxiliary subunit beta 3
Gene type protein-coding
Also known as CAB3, CACNLB3
Summary This gene encodes a regulatory beta subunit of the voltage-dependent calcium channel. Beta subunits are composed of five domains, which contribute to the regulation of surface expression and gating of calcium channels and may also play a role in the regulation of transcription factors and calcium transport.
Genomic regions Chromosome 12
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CACNB3-201 ENST00000301050.7 2931 484aa Protein coding CCDS8769 P54284-1 NM_000725.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CACNB3-204 ENST00000547230.5 2395 443aa Protein coding CCDS55823 P54284-5 - TSL:3, GENCODE basic,
CACNB3-202 ENST00000536187.6 1872 483aa Protein coding CCDS55822 P54284-4 - TSL:2, GENCODE basic,
CACNB3-203 ENST00000540990.5 1814 471aa Protein coding CCDS55821 P54284-3 - TSL:2, GENCODE basic,
CACNB3-205 ENST00000547392.5 2237 457aa Protein coding - F8VNV8 - TSL:5, GENCODE basic,
CACNB3-207 ENST00000547818.5 904 122aa Protein coding - F8VUW8 - CDS 3' incomplete, TSL:5,
CACNB3-213 ENST00000550064.5 746 55aa Protein coding - F8VU10 - CDS 3' incomplete, TSL:5,
CACNB3-208 ENST00000548279.5 576 144aa Protein coding - F8VWK1 - CDS 3' incomplete, TSL:4,
CACNB3-215 ENST00000550190.1 424 84aa Protein coding - H0YHK1 - CDS 5' incomplete, TSL:3,
CACNB3-219 ENST00000551544.5 2825 37aa Nonsense mediated decay - F8W1N3 - TSL:5,
CACNB3-216 ENST00000550391.5 754 151aa Nonsense mediated decay - F8VV14 - TSL:5,
CACNB3-217 ENST00000550483.5 730 76aa Nonsense mediated decay - H0YHY2 - CDS 5' incomplete, TSL:5,
CACNB3-210 ENST00000548874.5 581 105aa Nonsense mediated decay - F8W0F8 - TSL:3,
CACNB3-214 ENST00000550168.5 677 No protein Processed transcript - - - TSL:4,
CACNB3-221 ENST00000552022.5 564 No protein Processed transcript - - - TSL:4,
CACNB3-212 ENST00000549971.5 549 No protein Processed transcript - - - TSL:4,
CACNB3-209 ENST00000548860.5 4029 No protein Retained intron - - - TSL:2,
CACNB3-224 ENST00000552812.1 1712 No protein Retained intron - - - TSL:NA,
CACNB3-211 ENST00000549226.5 929 No protein Retained intron - - - TSL:2,
CACNB3-223 ENST00000552480.1 927 No protein Retained intron - - - TSL:3,
CACNB3-218 ENST00000550771.5 586 No protein Retained intron - - - TSL:4,
CACNB3-222 ENST00000552465.1 557 No protein Retained intron - - - TSL:3,
CACNB3-220 ENST00000551716.5 544 No protein Retained intron - - - TSL:3,
CACNB3-206 ENST00000547693.5 477 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CACNB3
This KO Strategy loading
Red Cotton™ Notes Gene CACNB3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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