CRISPR-U™ Gene Knockout Cell Line
Strategy
CACNB3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CACNB3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CACNB3 |
| Gene id | 784 |
| Organism | Homo sapiens |
| Official full symbol | calcium voltage-gated channel auxiliary subunit beta 3 |
| Gene type | protein-coding |
| Also known as | CAB3, CACNLB3 |
| Summary | This gene encodes a regulatory beta subunit of the voltage-dependent calcium channel. Beta subunits are composed of five domains, which contribute to the regulation of surface expression and gating of calcium channels and may also play a role in the regulation of transcription factors and calcium transport. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CACNB3-201 | ENST00000301050.7 | 2931 | 484aa | Protein coding | CCDS8769 | P54284-1 | NM_000725.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CACNB3-204 | ENST00000547230.5 | 2395 | 443aa | Protein coding | CCDS55823 | P54284-5 | - | TSL:3, GENCODE basic, |
| CACNB3-202 | ENST00000536187.6 | 1872 | 483aa | Protein coding | CCDS55822 | P54284-4 | - | TSL:2, GENCODE basic, |
| CACNB3-203 | ENST00000540990.5 | 1814 | 471aa | Protein coding | CCDS55821 | P54284-3 | - | TSL:2, GENCODE basic, |
| CACNB3-205 | ENST00000547392.5 | 2237 | 457aa | Protein coding | - | F8VNV8 | - | TSL:5, GENCODE basic, |
| CACNB3-207 | ENST00000547818.5 | 904 | 122aa | Protein coding | - | F8VUW8 | - | CDS 3' incomplete, TSL:5, |
| CACNB3-213 | ENST00000550064.5 | 746 | 55aa | Protein coding | - | F8VU10 | - | CDS 3' incomplete, TSL:5, |
| CACNB3-208 | ENST00000548279.5 | 576 | 144aa | Protein coding | - | F8VWK1 | - | CDS 3' incomplete, TSL:4, |
| CACNB3-215 | ENST00000550190.1 | 424 | 84aa | Protein coding | - | H0YHK1 | - | CDS 5' incomplete, TSL:3, |
| CACNB3-219 | ENST00000551544.5 | 2825 | 37aa | Nonsense mediated decay | - | F8W1N3 | - | TSL:5, |
| CACNB3-216 | ENST00000550391.5 | 754 | 151aa | Nonsense mediated decay | - | F8VV14 | - | TSL:5, |
| CACNB3-217 | ENST00000550483.5 | 730 | 76aa | Nonsense mediated decay | - | H0YHY2 | - | CDS 5' incomplete, TSL:5, |
| CACNB3-210 | ENST00000548874.5 | 581 | 105aa | Nonsense mediated decay | - | F8W0F8 | - | TSL:3, |
| CACNB3-214 | ENST00000550168.5 | 677 | No protein | Processed transcript | - | - | - | TSL:4, |
| CACNB3-221 | ENST00000552022.5 | 564 | No protein | Processed transcript | - | - | - | TSL:4, |
| CACNB3-212 | ENST00000549971.5 | 549 | No protein | Processed transcript | - | - | - | TSL:4, |
| CACNB3-209 | ENST00000548860.5 | 4029 | No protein | Retained intron | - | - | - | TSL:2, |
| CACNB3-224 | ENST00000552812.1 | 1712 | No protein | Retained intron | - | - | - | TSL:NA, |
| CACNB3-211 | ENST00000549226.5 | 929 | No protein | Retained intron | - | - | - | TSL:2, |
| CACNB3-223 | ENST00000552480.1 | 927 | No protein | Retained intron | - | - | - | TSL:3, |
| CACNB3-218 | ENST00000550771.5 | 586 | No protein | Retained intron | - | - | - | TSL:4, |
| CACNB3-222 | ENST00000552465.1 | 557 | No protein | Retained intron | - | - | - | TSL:3, |
| CACNB3-220 | ENST00000551716.5 | 544 | No protein | Retained intron | - | - | - | TSL:3, |
| CACNB3-206 | ENST00000547693.5 | 477 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CACNB3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CACNB3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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