CRISPR-U™ Gene Knockout Cell Line Strategy

CACNA2D1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CACNA2D1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CACNA2D1
Gene id 781
Organism Homo sapiens
Official full symbol calcium voltage-gated channel auxiliary subunit alpha2delta 1
Gene type protein-coding
Also known as CACNA2, CACNL2A, CCHL2A, LINC01112, lncRNA-N3
Summary The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion.
Genomic regions Chromosome 7
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CACNA2D1-202 ENST00000356860.8 7542 1091aa Protein coding CCDS5598 P54289-2 NM_000722.4 TSL:1, GENCODE basic, MANE Select v0.92,
CACNA2D1-203 ENST00000423588.1 1429 309aa Protein coding CCDS78253 E7ERK3 - TSL:1, GENCODE basic,
CACNA2D1-201 ENST00000356253.9 3858 1103aa Protein coding - P54289-1 - TSL:5, GENCODE basic, APPRIS P1,
CACNA2D1-204 ENST00000443883.1 840 280aa Protein coding - H0Y715 - CDS 5' and 3' incomplete, TSL:5,
CACNA2D1-206 ENST00000464354.5 582 No protein Processed transcript - - - TSL:3,
CACNA2D1-210 ENST00000484706.1 532 No protein Processed transcript - - - TSL:4,
CACNA2D1-205 ENST00000461275.1 467 No protein Processed transcript - - - TSL:3,
CACNA2D1-212 ENST00000492734.1 594 No protein Retained intron - - - TSL:3,
CACNA2D1-211 ENST00000486539.1 526 No protein Retained intron - - - TSL:5,
CACNA2D1-209 ENST00000475237.5 419 No protein Retained intron - - - TSL:3,
CACNA2D1-207 ENST00000466806.5 418 No protein Retained intron - - - TSL:3,
CACNA2D1-208 ENST00000469297.1 413 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CACNA2D1
This KO Strategy loading
Red Cotton™ Notes Gene CACNA2D1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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