CRISPR-U™ Gene Knockout Cell Line
Strategy
CACNA2D1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CACNA2D1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CACNA2D1 |
| Gene id | 781 |
| Organism | Homo sapiens |
| Official full symbol | calcium voltage-gated channel auxiliary subunit alpha2delta 1 |
| Gene type | protein-coding |
| Also known as | CACNA2, CACNL2A, CCHL2A, LINC01112, lncRNA-N3 |
| Summary | The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CACNA2D1-202 | ENST00000356860.8 | 7542 | 1091aa | Protein coding | CCDS5598 | P54289-2 | NM_000722.4 | TSL:1, GENCODE basic, MANE Select v0.92, |
| CACNA2D1-203 | ENST00000423588.1 | 1429 | 309aa | Protein coding | CCDS78253 | E7ERK3 | - | TSL:1, GENCODE basic, |
| CACNA2D1-201 | ENST00000356253.9 | 3858 | 1103aa | Protein coding | - | P54289-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CACNA2D1-204 | ENST00000443883.1 | 840 | 280aa | Protein coding | - | H0Y715 | - | CDS 5' and 3' incomplete, TSL:5, |
| CACNA2D1-206 | ENST00000464354.5 | 582 | No protein | Processed transcript | - | - | - | TSL:3, |
| CACNA2D1-210 | ENST00000484706.1 | 532 | No protein | Processed transcript | - | - | - | TSL:4, |
| CACNA2D1-205 | ENST00000461275.1 | 467 | No protein | Processed transcript | - | - | - | TSL:3, |
| CACNA2D1-212 | ENST00000492734.1 | 594 | No protein | Retained intron | - | - | - | TSL:3, |
| CACNA2D1-211 | ENST00000486539.1 | 526 | No protein | Retained intron | - | - | - | TSL:5, |
| CACNA2D1-209 | ENST00000475237.5 | 419 | No protein | Retained intron | - | - | - | TSL:3, |
| CACNA2D1-207 | ENST00000466806.5 | 418 | No protein | Retained intron | - | - | - | TSL:3, |
| CACNA2D1-208 | ENST00000469297.1 | 413 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CACNA2D1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CACNA2D1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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