CRISPR-U™ Gene Knockout Cell Line
Strategy
DDR1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DDR1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DDR1 |
| Gene id | 780 |
| Organism | Homo sapiens |
| Official full symbol | discoidin domain receptor tyrosine kinase 1 |
| Gene type | protein-coding |
| Also known as | CAK, CD167, DDR, EDDR1, HGK2, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE |
| Summary | Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 43 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DDR1-223 | ENST00000324771.12 | 4148 | 913aa | Protein coding | CCDS34385 | Q08345-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| DDR1-240 | ENST00000454612.6 | 3942 | 876aa | Protein coding | CCDS4690 | Q08345-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| DDR1-225 | ENST00000376568.7 | 3894 | 913aa | Protein coding | CCDS34385 | Q08345-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| DDR1-239 | ENST00000452441.5 | 3857 | 913aa | Protein coding | CCDS34385 | Q08345-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| DDR1-224 | ENST00000376567.6 | 3832 | 876aa | Protein coding | CCDS4690 | Q08345-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| DDR1-227 | ENST00000376570.8 | 3820 | 876aa | Protein coding | CCDS4690 | Q08345-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| DDR1-226 | ENST00000376569.7 | 3783 | 876aa | Protein coding | CCDS4690 | Q08345-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| DDR1-232 | ENST00000418800.6 | 3588 | 876aa | Protein coding | CCDS4690 | Q08345-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| DDR1-238 | ENST00000446312.5 | 3202 | 767aa | Protein coding | CCDS75419 | A0A0A0MSX3 | - | TSL:5, GENCODE basic, |
| DDR1-262 | ENST00000508312.5 | 3073 | 894aa | Protein coding | CCDS56411 | Q08345-6 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| DDR1-271 | ENST00000513240.5 | 2882 | 919aa | Protein coding | CCDS47396 | Q08345-5 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| DDR1-231 | ENST00000417521.5 | 1479 | 493aa | Protein coding | - | A2ABM8 | - | CDS 5' and 3' incomplete, TSL:5, |
| DDR1-235 | ENST00000428153.6 | 1257 | 267aa | Protein coding | - | E7EQ30 | - | CDS 3' incomplete, TSL:5, |
| DDR1-241 | ENST00000460944.6 | 1248 | 286aa | Protein coding | - | E7EPH4 | - | CDS 3' incomplete, TSL:5, |
| DDR1-245 | ENST00000484556.1 | 1038 | 277aa | Protein coding | - | H0Y9F4 | - | CDS 5' incomplete, TSL:2, |
| DDR1-280 | ENST00000515881.5 | 956 | 85aa | Protein coding | - | E7ENJ2 | - | CDS 3' incomplete, TSL:3, |
| DDR1-233 | ENST00000421124.6 | 800 | 209aa | Protein coding | - | A2ABL2 | - | CDS 3' incomplete, TSL:5, |
| DDR1-228 | ENST00000376575.7 | 732 | 243aa | Protein coding | - | Q08345-4 | - | TSL:1, GENCODE basic, |
| DDR1-229 | ENST00000396342.6 | 675 | 147aa | Protein coding | - | A2ABL0 | - | CDS 3' incomplete, TSL:3, |
| DDR1-257 | ENST00000505534.5 | 673 | 100aa | Protein coding | - | E7EN94 | - | CDS 3' incomplete, TSL:4, |
| DDR1-249 | ENST00000503495.5 | 636 | 196aa | Protein coding | - | E7EUP7 | - | CDS 3' incomplete, TSL:2, |
| DDR1-268 | ENST00000512694.5 | 623 | 170aa | Protein coding | - | E7EPN2 | - | CDS 3' incomplete, TSL:4, |
| DDR1-265 | ENST00000509639.5 | 615 | 114aa | Protein coding | - | E7ES06 | - | CDS 3' incomplete, TSL:4, |
| DDR1-270 | ENST00000513043.5 | 604 | 51aa | Protein coding | - | D6RB35 | - | CDS 3' incomplete, TSL:4, |
| DDR1-234 | ENST00000424544.2 | 587 | 196aa | Protein coding | - | H0Y717 | - | CDS 5' and 3' incomplete, TSL:4, |
| DDR1-237 | ENST00000437124.6 | 587 | 153aa | Protein coding | - | E7ERI6 | - | CDS 3' incomplete, TSL:4, |
| DDR1-278 | ENST00000515233.5 | 580 | 138aa | Protein coding | - | E7ERN0 | - | CDS 3' incomplete, TSL:4, |
| DDR1-247 | ENST00000502955.5 | 574 | 55aa | Protein coding | - | D6RB82 | - | CDS 3' incomplete, TSL:4, |
| DDR1-259 | ENST00000507046.5 | 573 | 70aa | Protein coding | - | E7EQ23 | - | CDS 3' incomplete, TSL:4, |
| DDR1-251 | ENST00000503670.5 | 572 | 41aa | Protein coding | - | D6R9C4 | - | CDS 3' incomplete, TSL:4, |
| DDR1-267 | ENST00000512336.5 | 572 | 112aa | Protein coding | - | E7EVT1 | - | CDS 3' incomplete, TSL:4, |
| DDR1-253 | ENST00000504651.5 | 571 | 73aa | Protein coding | - | E7EX99 | - | CDS 3' incomplete, TSL:3, |
| DDR1-261 | ENST00000507901.5 | 562 | 78aa | Protein coding | - | E7EXB0 | - | CDS 3' incomplete, TSL:4, |
| DDR1-256 | ENST00000505066.5 | 556 | 132aa | Protein coding | - | E7EVW6 | - | CDS 3' incomplete, TSL:4, |
| DDR1-266 | ENST00000511510.5 | 547 | 142aa | Protein coding | - | E7ESR9 | - | CDS 3' incomplete, TSL:5, |
| DDR1-255 | ENST00000504927.5 | 542 | 160aa | Protein coding | - | E7ETI3 | - | CDS 3' incomplete, TSL:4, |
| DDR1-269 | ENST00000512725.5 | 539 | 138aa | Protein coding | - | E7ERN0 | - | CDS 3' incomplete, TSL:4, |
| DDR1-263 | ENST00000508317.5 | 538 | 126aa | Protein coding | - | E7EUD5 | - | CDS 3' incomplete, TSL:4, |
| DDR1-248 | ENST00000503180.5 | 527 | 51aa | Protein coding | - | D6RB35 | - | CDS 3' incomplete, TSL:4, |
| DDR1-275 | ENST00000514434.1 | 514 | 172aa | Protein coding | - | H0YAH6 | - | CDS 5' and 3' incomplete, TSL:3, |
| DDR1-230 | ENST00000412274.6 | 496 | 58aa | Protein coding | - | D6RBU7 | - | CDS 3' incomplete, TSL:2, |
| DDR1-277 | ENST00000515219.5 | 481 | 69aa | Protein coding | - | D6RGW5 | - | CDS 3' incomplete, TSL:5, |
| DDR1-254 | ENST00000504679.5 | 460 | 122aa | Protein coding | - | E7ETX3 | - | CDS 3' incomplete, TSL:4, |
| DDR1-244 | ENST00000482873.6 | 2673 | 243aa | Nonsense mediated decay | - | Q08345-4 | - | TSL:5, |
| DDR1-274 | ENST00000513749.5 | 584 | 35aa | Nonsense mediated decay | - | D6RAJ3 | - | TSL:5, |
| DDR1-250 | ENST00000503628.5 | 570 | 35aa | Nonsense mediated decay | - | D6RAJ3 | - | TSL:4, |
| DDR1-264 | ENST00000508472.5 | 836 | No protein | Processed transcript | - | - | - | TSL:4, |
| DDR1-258 | ENST00000506573.1 | 585 | No protein | Processed transcript | - | - | - | TSL:4, |
| DDR1-279 | ENST00000515529.4 | 340 | No protein | Processed transcript | - | - | - | TSL:4, |
| DDR1-242 | ENST00000465966.4 | 268 | No protein | Processed transcript | - | - | - | TSL:1, |
| DDR1-243 | ENST00000482050.1 | 246 | No protein | Processed transcript | - | - | - | TSL:5, |
| DDR1-273 | ENST00000513514.2 | 186 | No protein | Processed transcript | - | - | - | TSL:1, |
| DDR1-236 | ENST00000431373.1 | 886 | No protein | Retained intron | - | - | - | TSL:2, |
| DDR1-252 | ENST00000504152.5 | 695 | No protein | Retained intron | - | - | - | TSL:3, |
| DDR1-276 | ENST00000514534.1 | 566 | No protein | Retained intron | - | - | - | TSL:3, |
| DDR1-272 | ENST00000513243.5 | 549 | No protein | Retained intron | - | - | - | TSL:5, |
| DDR1-260 | ENST00000507533.1 | 545 | No protein | Retained intron | - | - | - | TSL:2, |
| DDR1-246 | ENST00000485023.1 | 515 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DDR1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DDR1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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