CRISPR-U™ Gene Knockout Cell Line
Strategy
YES1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human YES1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | YES1 |
| Gene id | 7525 |
| Organism | Homo sapiens |
| Official full symbol | YES proto-oncogene 1, Src family tyrosine kinase |
| Gene type | protein-coding |
| Also known as | HsT441, P61-YES, Yes, c-yes |
| Summary | This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. |
| Genomic regions | Chromosome 18 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| YES1-205 | ENST00000584307.5 | 4639 | 543aa | Protein coding | CCDS11824 | P07947 | - | TSL:1, GENCODE basic, APPRIS P1, |
| YES1-201 | ENST00000314574.5 | 4605 | 543aa | Protein coding | CCDS11824 | P07947 | NM_005433.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| YES1-203 | ENST00000577961.5 | 4554 | 548aa | Protein coding | - | J3QRU1 | - | TSL:5, GENCODE basic, |
| YES1-202 | ENST00000577611.1 | 568 | No protein | Processed transcript | - | - | - | TSL:4, |
| YES1-204 | ENST00000581960.1 | 487 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | YES1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene YES1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: