CRISPR-U™ Gene Knockout Cell Line Strategy

YES1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human YES1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol YES1
Gene id 7525
Organism Homo sapiens
Official full symbol YES proto-oncogene 1, Src family tyrosine kinase
Gene type protein-coding
Also known as HsT441, P61-YES, Yes, c-yes
Summary This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.
Genomic regions Chromosome 18
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
YES1-205 ENST00000584307.5 4639 543aa Protein coding CCDS11824 P07947 - TSL:1, GENCODE basic, APPRIS P1,
YES1-201 ENST00000314574.5 4605 543aa Protein coding CCDS11824 P07947 NM_005433.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
YES1-203 ENST00000577961.5 4554 548aa Protein coding - J3QRU1 - TSL:5, GENCODE basic,
YES1-202 ENST00000577611.1 568 No protein Processed transcript - - - TSL:4,
YES1-204 ENST00000581960.1 487 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene YES1
This KO Strategy loading
Red Cotton™ Notes Gene YES1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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