CRISPR-U™ Gene Knockout Cell Line Strategy

VIM Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human VIM Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol VIM
Gene id 7431
Organism Homo sapiens
Official full symbol vimentin
Gene type protein-coding
Summary This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Genomic regions Chromosome 10
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
VIM-209 ENST00000544301.7 2154 466aa Protein coding CCDS7120 P08670 V9HWE1 NM_003380.5 TSL:1, APPRIS P1, MANE Select v0.92,
VIM-201 ENST00000224237.9 1868 466aa Protein coding CCDS7120 P08670 V9HWE1 - TSL:1, GENCODE basic, APPRIS P1,
VIM-204 ENST00000478746.1 757 149aa Protein coding - A0A1B0GTT5 - CDS 3' incomplete, TSL:2,
VIM-208 ENST00000497849.1 634 109aa Protein coding - A0A1B0GVG8 - CDS 3' incomplete, TSL:2,
VIM-203 ENST00000469543.5 2666 228aa Nonsense mediated decay - B0YJC5 - TSL:2,
VIM-206 ENST00000487938.5 2272 431aa Nonsense mediated decay - B0YJC4 - TSL:5,
VIM-205 ENST00000485947.1 1139 No protein Processed transcript - - - TSL:2,
VIM-210 ENST00000637053.1 508 No protein Processed transcript - - - TSL:2,
VIM-207 ENST00000495528.1 962 No protein Retained intron - - - TSL:2,
VIM-202 ENST00000421459.2 753 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene VIM
This KO Strategy loading
Red Cotton™ Notes Gene VIM had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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