CRISPR-U™ Gene Knockout Cell Line
Strategy
VIM Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human VIM Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | VIM |
| Gene id | 7431 |
| Organism | Homo sapiens |
| Official full symbol | vimentin |
| Gene type | protein-coding |
| Summary | This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| VIM-209 | ENST00000544301.7 | 2154 | 466aa | Protein coding | CCDS7120 | P08670 V9HWE1 | NM_003380.5 | TSL:1, APPRIS P1, MANE Select v0.92, |
| VIM-201 | ENST00000224237.9 | 1868 | 466aa | Protein coding | CCDS7120 | P08670 V9HWE1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| VIM-204 | ENST00000478746.1 | 757 | 149aa | Protein coding | - | A0A1B0GTT5 | - | CDS 3' incomplete, TSL:2, |
| VIM-208 | ENST00000497849.1 | 634 | 109aa | Protein coding | - | A0A1B0GVG8 | - | CDS 3' incomplete, TSL:2, |
| VIM-203 | ENST00000469543.5 | 2666 | 228aa | Nonsense mediated decay | - | B0YJC5 | - | TSL:2, |
| VIM-206 | ENST00000487938.5 | 2272 | 431aa | Nonsense mediated decay | - | B0YJC4 | - | TSL:5, |
| VIM-205 | ENST00000485947.1 | 1139 | No protein | Processed transcript | - | - | - | TSL:2, |
| VIM-210 | ENST00000637053.1 | 508 | No protein | Processed transcript | - | - | - | TSL:2, |
| VIM-207 | ENST00000495528.1 | 962 | No protein | Retained intron | - | - | - | TSL:2, |
| VIM-202 | ENST00000421459.2 | 753 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | VIM |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene VIM had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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