CRISPR-U™ Gene Knockout Cell Line
Strategy
VDR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human VDR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | VDR |
| Gene id | 7421 |
| Organism | Homo sapiens |
| Official full symbol | vitamin D receptor |
| Gene type | protein-coding |
| Also known as | NR1I1, PPP1R163 |
| Summary | This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| VDR-202 | ENST00000395324.6 | 4762 | 427aa | Protein coding | CCDS8757 | P11473-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| VDR-206 | ENST00000549336.6 | 4616 | 427aa | Protein coding | CCDS8757 | P11473-1 | NM_000376.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| VDR-208 | ENST00000550325.5 | 3925 | 477aa | Protein coding | CCDS55820 | P11473-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| VDR-201 | ENST00000229022.9 | 3404 | 494aa | Protein coding | - | A0A5K1VW50 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| VDR-203 | ENST00000546653.5 | 937 | 250aa | Protein coding | - | F8VRJ4 | - | CDS 3' incomplete, TSL:5, |
| VDR-205 | ENST00000548664.1 | 596 | 101aa | Protein coding | - | F8VPF8 | - | CDS 3' incomplete, TSL:5, |
| VDR-207 | ENST00000550314.5 | 562 | 106aa | Protein coding | - | F8VVY8 | - | CDS 3' incomplete, TSL:3, |
| VDR-204 | ENST00000547065.1 | 1782 | 49aa | Nonsense mediated decay | - | F8VXQ9 | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | VDR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene VDR had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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