CRISPR-U™ Gene Knockout Cell Line Strategy

VDR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human VDR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol VDR
Gene id 7421
Organism Homo sapiens
Official full symbol vitamin D receptor
Gene type protein-coding
Also known as NR1I1, PPP1R163
Summary This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
Genomic regions Chromosome 12
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
VDR-202 ENST00000395324.6 4762 427aa Protein coding CCDS8757 P11473-1 - TSL:5, GENCODE basic, APPRIS P4,
VDR-206 ENST00000549336.6 4616 427aa Protein coding CCDS8757 P11473-1 NM_000376.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
VDR-208 ENST00000550325.5 3925 477aa Protein coding CCDS55820 P11473-2 - TSL:1, GENCODE basic, APPRIS ALT2,
VDR-201 ENST00000229022.9 3404 494aa Protein coding - A0A5K1VW50 - TSL:5, GENCODE basic, APPRIS ALT2,
VDR-203 ENST00000546653.5 937 250aa Protein coding - F8VRJ4 - CDS 3' incomplete, TSL:5,
VDR-205 ENST00000548664.1 596 101aa Protein coding - F8VPF8 - CDS 3' incomplete, TSL:5,
VDR-207 ENST00000550314.5 562 106aa Protein coding - F8VVY8 - CDS 3' incomplete, TSL:3,
VDR-204 ENST00000547065.1 1782 49aa Nonsense mediated decay - F8VXQ9 - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene VDR
This KO Strategy loading
Red Cotton™ Notes Gene VDR had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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