CRISPR-U™ Gene Knockout Cell Line
Strategy
VDAC1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human VDAC1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | VDAC1 |
| Gene id | 7416 |
| Organism | Homo sapiens |
| Official full symbol | voltage dependent anion channel 1 |
| Gene type | protein-coding |
| Also known as | PORIN, VDAC-1 |
| Summary | This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| VDAC1-203 | ENST00000395047.6 | 1873 | 283aa | Protein coding | CCDS4168 | A0A1L1UHR1 P21796 | - | TSL:1, GENCODE basic, APPRIS P1, |
| VDAC1-201 | ENST00000265333.8 | 1839 | 283aa | Protein coding | CCDS4168 | A0A1L1UHR1 P21796 | NM_003374.3 | TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| VDAC1-202 | ENST00000395044.7 | 1807 | 283aa | Protein coding | CCDS4168 | A0A1L1UHR1 P21796 | - | TSL:1, GENCODE basic, APPRIS P1, |
| VDAC1-204 | ENST00000425992.2 | 703 | 183aa | Protein coding | - | C9JI87 | - | CDS 3' incomplete, TSL:3, |
| VDAC1-205 | ENST00000466080.1 | 831 | No protein | Processed transcript | - | - | - | TSL:3, |
| VDAC1-207 | ENST00000492324.1 | 635 | No protein | Processed transcript | - | - | - | TSL:2, |
| VDAC1-206 | ENST00000489906.5 | 694 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | VDAC1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene VDAC1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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