CRISPR-U™ Gene Knockout Cell Line Strategy

VDAC1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human VDAC1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol VDAC1
Gene id 7416
Organism Homo sapiens
Official full symbol voltage dependent anion channel 1
Gene type protein-coding
Also known as PORIN, VDAC-1
Summary This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.
Genomic regions Chromosome 5
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
VDAC1-203 ENST00000395047.6 1873 283aa Protein coding CCDS4168 A0A1L1UHR1 P21796 - TSL:1, GENCODE basic, APPRIS P1,
VDAC1-201 ENST00000265333.8 1839 283aa Protein coding CCDS4168 A0A1L1UHR1 P21796 NM_003374.3 TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92,
VDAC1-202 ENST00000395044.7 1807 283aa Protein coding CCDS4168 A0A1L1UHR1 P21796 - TSL:1, GENCODE basic, APPRIS P1,
VDAC1-204 ENST00000425992.2 703 183aa Protein coding - C9JI87 - CDS 3' incomplete, TSL:3,
VDAC1-205 ENST00000466080.1 831 No protein Processed transcript - - - TSL:3,
VDAC1-207 ENST00000492324.1 635 No protein Processed transcript - - - TSL:2,
VDAC1-206 ENST00000489906.5 694 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene VDAC1
This KO Strategy loading
Red Cotton™ Notes Gene VDAC1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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