CRISPR-U™ Gene Knockout Cell Line Strategy

VCL Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human VCL Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol VCL
Gene id 7414
Organism Homo sapiens
Official full symbol vinculin
Gene type protein-coding
Also known as CMD1W, CMH15, HEL114, MV, MVCL
Summary Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Genomic regions Chromosome 10
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
VCL-202 ENST00000372755.7 6489 1066aa Protein coding CCDS7340 P18206-2 - TSL:1, GENCODE basic, APPRIS P4,
VCL-201 ENST00000211998.10 5497 1134aa Protein coding CCDS7341 P18206-1 NM_014000.3 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
VCL-207 ENST00000624354.3 3307 57aa Nonsense mediated decay - A0A096LPE1 - TSL:2,
VCL-206 ENST00000623461.3 7995 No protein Processed transcript - - - TSL:1,
VCL-205 ENST00000478896.2 727 No protein Processed transcript - - - TSL:5,
VCL-203 ENST00000461383.1 268 No protein Processed transcript - - - TSL:2,
VCL-204 ENST00000472585.1 556 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene VCL
This KO Strategy loading
Red Cotton™ Notes Gene VCL had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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