CRISPR-U™ Gene Knockout Cell Line
Strategy
VCL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human VCL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | VCL |
| Gene id | 7414 |
| Organism | Homo sapiens |
| Official full symbol | vinculin |
| Gene type | protein-coding |
| Also known as | CMD1W, CMH15, HEL114, MV, MVCL |
| Summary | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| VCL-202 | ENST00000372755.7 | 6489 | 1066aa | Protein coding | CCDS7340 | P18206-2 | - | TSL:1, GENCODE basic, APPRIS P4, |
| VCL-201 | ENST00000211998.10 | 5497 | 1134aa | Protein coding | CCDS7341 | P18206-1 | NM_014000.3 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| VCL-207 | ENST00000624354.3 | 3307 | 57aa | Nonsense mediated decay | - | A0A096LPE1 | - | TSL:2, |
| VCL-206 | ENST00000623461.3 | 7995 | No protein | Processed transcript | - | - | - | TSL:1, |
| VCL-205 | ENST00000478896.2 | 727 | No protein | Processed transcript | - | - | - | TSL:5, |
| VCL-203 | ENST00000461383.1 | 268 | No protein | Processed transcript | - | - | - | TSL:2, |
| VCL-204 | ENST00000472585.1 | 556 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | VCL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene VCL had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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