CRISPR-U™ Gene Knockout Cell Line
Strategy
VAV2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human VAV2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | VAV2 |
| Gene id | 7410 |
| Organism | Homo sapiens |
| Official full symbol | vav guanine nucleotide exchange factor 2 |
| Gene type | protein-coding |
| Also known as | VAV-2 |
| Summary | VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| VAV2-201 | ENST00000371850.8 | 4851 | 878aa | Protein coding | CCDS48053 | P52735-1 | NM_001134398.2 | TSL:1, GENCODE basic, MANE Select v0.92, |
| VAV2-203 | ENST00000406606.7 | 4691 | 839aa | Protein coding | CCDS6979 | P52735-3 | - | TSL:1, GENCODE basic, APPRIS P4, |
| VAV2-202 | ENST00000371851.1 | 5101 | 868aa | Protein coding | - | P52735-2 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| VAV2-205 | ENST00000486113.1 | 523 | No protein | Processed transcript | - | - | - | TSL:2, |
| VAV2-204 | ENST00000472905.1 | 506 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | VAV2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene VAV2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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