CRISPR-U™ Gene Knockout Cell Line Strategy

VAV2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human VAV2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol VAV2
Gene id 7410
Organism Homo sapiens
Official full symbol vav guanine nucleotide exchange factor 2
Gene type protein-coding
Also known as VAV-2
Summary VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 9
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
VAV2-201 ENST00000371850.8 4851 878aa Protein coding CCDS48053 P52735-1 NM_001134398.2 TSL:1, GENCODE basic, MANE Select v0.92,
VAV2-203 ENST00000406606.7 4691 839aa Protein coding CCDS6979 P52735-3 - TSL:1, GENCODE basic, APPRIS P4,
VAV2-202 ENST00000371851.1 5101 868aa Protein coding - P52735-2 - TSL:5, GENCODE basic, APPRIS ALT1,
VAV2-205 ENST00000486113.1 523 No protein Processed transcript - - - TSL:2,
VAV2-204 ENST00000472905.1 506 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene VAV2
This KO Strategy loading
Red Cotton™ Notes Gene VAV2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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