CRISPR-U™ Gene Knockout Cell Line Strategy

VASP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human VASP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol VASP
Gene id 7408
Organism Homo sapiens
Official full symbol vasodilator stimulated phosphoprotein
Gene type protein-coding
Summary Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Genomic regions Chromosome 19
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
VASP-201 ENST00000245932.11 2242 380aa Protein coding CCDS33051 A0A024R0V4 P50552 NM_003370.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
VASP-208 ENST00000589627.1 696 59aa Protein coding - K7ENR7 - CDS 3' incomplete, TSL:3,
VASP-211 ENST00000592139.1 424 46aa Protein coding - K7EIG8 - CDS 3' incomplete, TSL:3,
VASP-210 ENST00000590603.1 388 35aa Protein coding - K7EQD0 - CDS 3' incomplete, TSL:2,
VASP-202 ENST00000586014.5 1725 103aa Nonsense mediated decay - K7ENL7 - TSL:5,
VASP-207 ENST00000588482.5 804 183aa Nonsense mediated decay - K7EM16 - CDS 5' incomplete, TSL:5,
VASP-203 ENST00000586619.1 655 No protein Processed transcript - - - TSL:2,
VASP-206 ENST00000588463.1 655 No protein Retained intron - - - TSL:2,
VASP-209 ENST00000590459.1 405 No protein Retained intron - - - TSL:2,
VASP-205 ENST00000588273.1 318 No protein Retained intron - - - TSL:2,
VASP-204 ENST00000587444.1 240 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene VASP
This KO Strategy loading
Red Cotton™ Notes Gene VASP had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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