CRISPR-U™ Gene Knockout Cell Line
Strategy
VASP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human VASP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | VASP |
| Gene id | 7408 |
| Organism | Homo sapiens |
| Official full symbol | vasodilator stimulated phosphoprotein |
| Gene type | protein-coding |
| Summary | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| VASP-201 | ENST00000245932.11 | 2242 | 380aa | Protein coding | CCDS33051 | A0A024R0V4 P50552 | NM_003370.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| VASP-208 | ENST00000589627.1 | 696 | 59aa | Protein coding | - | K7ENR7 | - | CDS 3' incomplete, TSL:3, |
| VASP-211 | ENST00000592139.1 | 424 | 46aa | Protein coding | - | K7EIG8 | - | CDS 3' incomplete, TSL:3, |
| VASP-210 | ENST00000590603.1 | 388 | 35aa | Protein coding | - | K7EQD0 | - | CDS 3' incomplete, TSL:2, |
| VASP-202 | ENST00000586014.5 | 1725 | 103aa | Nonsense mediated decay | - | K7ENL7 | - | TSL:5, |
| VASP-207 | ENST00000588482.5 | 804 | 183aa | Nonsense mediated decay | - | K7EM16 | - | CDS 5' incomplete, TSL:5, |
| VASP-203 | ENST00000586619.1 | 655 | No protein | Processed transcript | - | - | - | TSL:2, |
| VASP-206 | ENST00000588463.1 | 655 | No protein | Retained intron | - | - | - | TSL:2, |
| VASP-209 | ENST00000590459.1 | 405 | No protein | Retained intron | - | - | - | TSL:2, |
| VASP-205 | ENST00000588273.1 | 318 | No protein | Retained intron | - | - | - | TSL:2, |
| VASP-204 | ENST00000587444.1 | 240 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | VASP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene VASP had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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