CRISPR-U™ Gene Knockout Cell Line
Strategy
UCHL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human UCHL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | UCHL1 |
| Gene id | 7345 |
| Organism | Homo sapiens |
| Official full symbol | ubiquitin C-terminal hydrolase L1 |
| Gene type | protein-coding |
| Also known as | HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1 |
| Summary | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| UCHL1-201 | ENST00000284440.9 | 1103 | 223aa | Protein coding | CCDS3462 | P09936 V9HW74 | NM_004181.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| UCHL1-204 | ENST00000503431.5 | 917 | 223aa | Protein coding | CCDS3462 | P09936 V9HW74 | - | TSL:3, GENCODE basic, APPRIS P1, |
| UCHL1-207 | ENST00000508768.5 | 827 | 207aa | Protein coding | - | D6RE83 | - | TSL:3, GENCODE basic, |
| UCHL1-210 | ENST00000512788.1 | 785 | 241aa | Protein coding | - | D6R956 | - | TSL:3, GENCODE basic, |
| UCHL1-212 | ENST00000514924.5 | 445 | 58aa | Protein coding | - | D6RF53 | - | CDS 3' incomplete, TSL:3, |
| UCHL1-209 | ENST00000512419.5 | 1119 | 69aa | Nonsense mediated decay | - | D6RJD9 | - | TSL:5, |
| UCHL1-206 | ENST00000505232.5 | 748 | 156aa | Nonsense mediated decay | - | D6R974 | - | TSL:3, |
| UCHL1-205 | ENST00000504818.5 | 726 | No protein | Processed transcript | - | - | - | TSL:5, |
| UCHL1-202 | ENST00000381760.8 | 1578 | No protein | Retained intron | - | - | - | TSL:2, |
| UCHL1-203 | ENST00000472501.5 | 1550 | No protein | Retained intron | - | - | - | TSL:5, |
| UCHL1-211 | ENST00000514764.5 | 862 | No protein | Retained intron | - | - | - | TSL:2, |
| UCHL1-208 | ENST00000510566.1 | 540 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | UCHL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene UCHL1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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