CRISPR-U™ Gene Knockout Cell Line Strategy

UCHL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human UCHL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol UCHL1
Gene id 7345
Organism Homo sapiens
Official full symbol ubiquitin C-terminal hydrolase L1
Gene type protein-coding
Also known as HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1
Summary The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
Genomic regions Chromosome 4
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
UCHL1-201 ENST00000284440.9 1103 223aa Protein coding CCDS3462 P09936 V9HW74 NM_004181.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
UCHL1-204 ENST00000503431.5 917 223aa Protein coding CCDS3462 P09936 V9HW74 - TSL:3, GENCODE basic, APPRIS P1,
UCHL1-207 ENST00000508768.5 827 207aa Protein coding - D6RE83 - TSL:3, GENCODE basic,
UCHL1-210 ENST00000512788.1 785 241aa Protein coding - D6R956 - TSL:3, GENCODE basic,
UCHL1-212 ENST00000514924.5 445 58aa Protein coding - D6RF53 - CDS 3' incomplete, TSL:3,
UCHL1-209 ENST00000512419.5 1119 69aa Nonsense mediated decay - D6RJD9 - TSL:5,
UCHL1-206 ENST00000505232.5 748 156aa Nonsense mediated decay - D6R974 - TSL:3,
UCHL1-205 ENST00000504818.5 726 No protein Processed transcript - - - TSL:5,
UCHL1-202 ENST00000381760.8 1578 No protein Retained intron - - - TSL:2,
UCHL1-203 ENST00000472501.5 1550 No protein Retained intron - - - TSL:5,
UCHL1-211 ENST00000514764.5 862 No protein Retained intron - - - TSL:2,
UCHL1-208 ENST00000510566.1 540 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene UCHL1
This KO Strategy loading
Red Cotton™ Notes Gene UCHL1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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