CRISPR-U™ Gene Knockout Cell Line Strategy

TSN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TSN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TSN
Gene id 7247
Organism Homo sapiens
Official full symbol translin
Gene type protein-coding
Also known as BCLF-1, C3PO, RCHF1, REHF-1, TBRBP, TRSLN
Summary This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. This protein encoded by this gene, when complexed with translin-associated protein X, also forms a Mg ion-dependent endoribonuclease that promotes RNA-induced silencing complex (RISC) activation. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 2
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TSN-212 ENST00000536142.5 3328 148aa Protein coding CCDS58723 Q15631-2 - TSL:2, GENCODE basic,
TSN-201 ENST00000389682.8 3302 228aa Protein coding CCDS33284 Q15631-1 NM_004622.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TSN-202 ENST00000409193.1 1436 223aa Protein coding - E9PGT1 - TSL:5, GENCODE basic,
TSN-203 ENST00000455432.5 555 185aa Protein coding - H7C1D4 - CDS 5' and 3' incomplete, TSL:4,
TSN-204 ENST00000467324.5 2843 36aa Nonsense mediated decay - A0A0J9YX41 - TSL:5,
TSN-208 ENST00000490104.5 2717 92aa Nonsense mediated decay - A0A0J9YVW2 - TSL:2,
TSN-205 ENST00000478165.5 578 36aa Nonsense mediated decay - A0A0J9YX41 - TSL:3,
TSN-209 ENST00000490717.5 560 36aa Nonsense mediated decay - A0A0J9YX41 - TSL:4,
TSN-211 ENST00000498545.5 1118 No protein Processed transcript - - - TSL:1,
TSN-210 ENST00000495112.1 605 No protein Processed transcript - - - TSL:4,
TSN-207 ENST00000483698.5 555 No protein Retained intron - - - TSL:2,
TSN-206 ENST00000481818.1 421 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TSN
This KO Strategy loading
Red Cotton™ Notes Gene TSN had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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