CRISPR-U™ Gene Knockout Cell Line
Strategy
TSN Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TSN Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TSN |
| Gene id | 7247 |
| Organism | Homo sapiens |
| Official full symbol | translin |
| Gene type | protein-coding |
| Also known as | BCLF-1, C3PO, RCHF1, REHF-1, TBRBP, TRSLN |
| Summary | This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. This protein encoded by this gene, when complexed with translin-associated protein X, also forms a Mg ion-dependent endoribonuclease that promotes RNA-induced silencing complex (RISC) activation. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TSN-212 | ENST00000536142.5 | 3328 | 148aa | Protein coding | CCDS58723 | Q15631-2 | - | TSL:2, GENCODE basic, |
| TSN-201 | ENST00000389682.8 | 3302 | 228aa | Protein coding | CCDS33284 | Q15631-1 | NM_004622.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TSN-202 | ENST00000409193.1 | 1436 | 223aa | Protein coding | - | E9PGT1 | - | TSL:5, GENCODE basic, |
| TSN-203 | ENST00000455432.5 | 555 | 185aa | Protein coding | - | H7C1D4 | - | CDS 5' and 3' incomplete, TSL:4, |
| TSN-204 | ENST00000467324.5 | 2843 | 36aa | Nonsense mediated decay | - | A0A0J9YX41 | - | TSL:5, |
| TSN-208 | ENST00000490104.5 | 2717 | 92aa | Nonsense mediated decay | - | A0A0J9YVW2 | - | TSL:2, |
| TSN-205 | ENST00000478165.5 | 578 | 36aa | Nonsense mediated decay | - | A0A0J9YX41 | - | TSL:3, |
| TSN-209 | ENST00000490717.5 | 560 | 36aa | Nonsense mediated decay | - | A0A0J9YX41 | - | TSL:4, |
| TSN-211 | ENST00000498545.5 | 1118 | No protein | Processed transcript | - | - | - | TSL:1, |
| TSN-210 | ENST00000495112.1 | 605 | No protein | Processed transcript | - | - | - | TSL:4, |
| TSN-207 | ENST00000483698.5 | 555 | No protein | Retained intron | - | - | - | TSL:2, |
| TSN-206 | ENST00000481818.1 | 421 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TSN |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TSN had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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