CRISPR-U™ Gene Knockout Cell Line
Strategy
NR2C2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NR2C2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NR2C2 |
| Gene id | 7182 |
| Organism | Homo sapiens |
| Official full symbol | nuclear receptor subfamily 2 group C member 2 |
| Gene type | protein-coding |
| Also known as | TAK1, TR4 |
| Summary | This gene encodes a protein that belongs to the nuclear hormone receptor family. Members of this family act as ligand-activated transcription factors and function in many biological processes such as development, cellular differentiation and homeostasis. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes. The protein encoded by this gene plays a role in protecting cells from oxidative stress and damage induced by ionizing radiation. The lack of a similar gene in mouse results in growth retardation, severe spinal curvature, subfertility, premature aging, and prostatic intraepithelial neoplasia (PIN) development. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NR2C2-213 | ENST00000617312.4 | 8113 | 615aa | Protein coding | CCDS2621 | P49116-2 | - | TSL:1, GENCODE basic, |
| NR2C2-206 | ENST00000425241.5 | 2494 | 596aa | Protein coding | CCDS74905 | P49116-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| NR2C2-201 | ENST00000323373.10 | 2406 | 615aa | Protein coding | CCDS2621 | P49116-2 | - | TSL:1, GENCODE basic, |
| NR2C2-202 | ENST00000393102.7 | 1937 | 596aa | Protein coding | CCDS74905 | P49116-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| NR2C2-203 | ENST00000406272.6 | 1791 | 596aa | Protein coding | CCDS74905 | P49116-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NR2C2-208 | ENST00000437120.5 | 739 | 205aa | Protein coding | - | C9JKS0 | - | CDS 3' incomplete, TSL:3, |
| NR2C2-209 | ENST00000439011.5 | 698 | 222aa | Protein coding | - | H7C3L1 | - | CDS 5' incomplete, TSL:3, |
| NR2C2-204 | ENST00000413118.5 | 564 | 67aa | Protein coding | - | C9JMI6 | - | CDS 3' incomplete, TSL:4, |
| NR2C2-205 | ENST00000413194.1 | 551 | 173aa | Protein coding | - | H7C3R1 | - | CDS 5' incomplete, TSL:3, |
| NR2C2-207 | ENST00000435454.5 | 495 | 36aa | Protein coding | - | C9J2Y1 | - | CDS 3' incomplete, TSL:3, |
| NR2C2-211 | ENST00000478572.1 | 2767 | No protein | Processed transcript | - | - | - | TSL:2, |
| NR2C2-210 | ENST00000475707.5 | 3308 | No protein | Retained intron | - | - | - | TSL:2, |
| NR2C2-212 | ENST00000495282.1 | 534 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NR2C2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NR2C2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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