CRISPR-U™ Gene Knockout Cell Line Strategy

NR2C2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NR2C2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NR2C2
Gene id 7182
Organism Homo sapiens
Official full symbol nuclear receptor subfamily 2 group C member 2
Gene type protein-coding
Also known as TAK1, TR4
Summary This gene encodes a protein that belongs to the nuclear hormone receptor family. Members of this family act as ligand-activated transcription factors and function in many biological processes such as development, cellular differentiation and homeostasis. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes. The protein encoded by this gene plays a role in protecting cells from oxidative stress and damage induced by ionizing radiation. The lack of a similar gene in mouse results in growth retardation, severe spinal curvature, subfertility, premature aging, and prostatic intraepithelial neoplasia (PIN) development. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 3
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NR2C2-213 ENST00000617312.4 8113 615aa Protein coding CCDS2621 P49116-2 - TSL:1, GENCODE basic,
NR2C2-206 ENST00000425241.5 2494 596aa Protein coding CCDS74905 P49116-1 - TSL:2, GENCODE basic, APPRIS P1,
NR2C2-201 ENST00000323373.10 2406 615aa Protein coding CCDS2621 P49116-2 - TSL:1, GENCODE basic,
NR2C2-202 ENST00000393102.7 1937 596aa Protein coding CCDS74905 P49116-1 - TSL:5, GENCODE basic, APPRIS P1,
NR2C2-203 ENST00000406272.6 1791 596aa Protein coding CCDS74905 P49116-1 - TSL:1, GENCODE basic, APPRIS P1,
NR2C2-208 ENST00000437120.5 739 205aa Protein coding - C9JKS0 - CDS 3' incomplete, TSL:3,
NR2C2-209 ENST00000439011.5 698 222aa Protein coding - H7C3L1 - CDS 5' incomplete, TSL:3,
NR2C2-204 ENST00000413118.5 564 67aa Protein coding - C9JMI6 - CDS 3' incomplete, TSL:4,
NR2C2-205 ENST00000413194.1 551 173aa Protein coding - H7C3R1 - CDS 5' incomplete, TSL:3,
NR2C2-207 ENST00000435454.5 495 36aa Protein coding - C9J2Y1 - CDS 3' incomplete, TSL:3,
NR2C2-211 ENST00000478572.1 2767 No protein Processed transcript - - - TSL:2,
NR2C2-210 ENST00000475707.5 3308 No protein Retained intron - - - TSL:2,
NR2C2-212 ENST00000495282.1 534 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NR2C2
This KO Strategy loading
Red Cotton™ Notes Gene NR2C2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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