CRISPR-U™ Gene Knockout Cell Line
Strategy
TPM4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TPM4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TPM4 |
| Gene id | 7171 |
| Organism | Homo sapiens |
| Official full symbol | tropomyosin 4 |
| Gene type | protein-coding |
| Also known as | HEL-S-108 |
| Summary | This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TPM4-221 | ENST00000646974.2 | 2598 | 284aa | Protein coding | CCDS46007 | P67936-2 | - | GENCODE basic, APPRIS P1, |
| TPM4-218 | ENST00000643579.2 | 2474 | 248aa | Protein coding | CCDS12338 | P67936-1 | NM_003290.3 | GENCODE basic, MANE Select v0.92, |
| TPM4-215 | ENST00000642221.1 | 2567 | 226aa | Protein coding | - | A0A2R8YE05 | - | GENCODE basic, |
| TPM4-205 | ENST00000586833.7 | 2491 | 245aa | Protein coding | - | K7ENT6 | - | TSL:5, GENCODE basic, |
| TPM4-223 | ENST00000647464.2 | 2370 | 248aa | Protein coding | - | A0A2R8YHD2 | - | CDS 5' incomplete, |
| TPM4-202 | ENST00000344824.11 | 2359 | 158aa | Protein coding | - | A0A5F9UN72 | - | TSL:5, GENCODE basic, |
| TPM4-220 | ENST00000646575.1 | 2355 | 220aa | Protein coding | - | A0A2R8YH90 | - | CDS 5' incomplete, |
| TPM4-210 | ENST00000589897.1 | 586 | 69aa | Protein coding | - | K7ELP0 | - | CDS 3' incomplete, TSL:4, |
| TPM4-219 | ENST00000645471.1 | 3106 | 248aa | Nonsense mediated decay | - | A0A2R8Y5V9 | - | - |
| TPM4-222 | ENST00000647037.1 | 2927 | 248aa | Nonsense mediated decay | - | A0A2R8Y5V9 | - | - |
| TPM4-204 | ENST00000586499.6 | 2638 | 74aa | Nonsense mediated decay | - | K7ERG3 | - | CDS 5' incomplete, TSL:4, |
| TPM4-235 | ENST00000670382.1 | 2551 | 44aa | Nonsense mediated decay | - | A0A590UJB9 | - | - |
| TPM4-217 | ENST00000643494.1 | 2504 | 183aa | Nonsense mediated decay | - | A0A2R8YGX3 | - | - |
| TPM4-216 | ENST00000642789.1 | 2431 | 173aa | Nonsense mediated decay | - | A0A2R8YEU4 | - | CDS 5' incomplete, |
| TPM4-201 | ENST00000300933.9 | 2330 | 83aa | Nonsense mediated decay | - | A0A5F9UKJ4 | - | CDS 5' incomplete, TSL:1, |
| TPM4-231 | ENST00000659595.1 | 2330 | 56aa | Nonsense mediated decay | - | A0A590UK79 | - | - |
| TPM4-225 | ENST00000653979.1 | 2163 | 40aa | Nonsense mediated decay | - | A0A590UKD0 | - | CDS 5' incomplete, |
| TPM4-207 | ENST00000588032.6 | 707 | 110aa | Nonsense mediated decay | - | K7EPB9 | - | CDS 5' incomplete, TSL:3, |
| TPM4-209 | ENST00000588483.2 | 2867 | No protein | Processed transcript | - | - | - | TSL:5, |
| TPM4-228 | ENST00000657915.1 | 2805 | No protein | Processed transcript | - | - | - | - |
| TPM4-234 | ENST00000668959.1 | 2540 | No protein | Processed transcript | - | - | - | - |
| TPM4-211 | ENST00000590180.3 | 2364 | No protein | Processed transcript | - | - | - | TSL:3, |
| TPM4-213 | ENST00000591645.3 | 2167 | No protein | Processed transcript | - | - | - | TSL:3, |
| TPM4-230 | ENST00000658489.1 | 1974 | No protein | Processed transcript | - | - | - | - |
| TPM4-224 | ENST00000653961.1 | 1879 | No protein | Processed transcript | - | - | - | - |
| TPM4-229 | ENST00000658224.1 | 4894 | No protein | Retained intron | - | - | - | - |
| TPM4-203 | ENST00000586193.6 | 3369 | No protein | Retained intron | - | - | - | TSL:3, |
| TPM4-212 | ENST00000591226.2 | 2675 | No protein | Retained intron | - | - | - | TSL:NA, |
| TPM4-233 | ENST00000664983.1 | 2582 | No protein | Retained intron | - | - | - | - |
| TPM4-227 | ENST00000655004.1 | 2447 | No protein | Retained intron | - | - | - | - |
| TPM4-232 | ENST00000663894.1 | 2266 | No protein | Retained intron | - | - | - | - |
| TPM4-206 | ENST00000587201.6 | 1879 | No protein | Retained intron | - | - | - | TSL:2, |
| TPM4-214 | ENST00000592138.6 | 1396 | No protein | Retained intron | - | - | - | TSL:2, |
| TPM4-208 | ENST00000588410.2 | 1283 | No protein | Retained intron | - | - | - | TSL:4, |
| TPM4-226 | ENST00000654417.1 | 1011 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TPM4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TPM4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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