CRISPR-U™ Gene Knockout Cell Line
Strategy
TPM3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TPM3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TPM3 |
| Gene id | 7170 |
| Organism | Homo sapiens |
| Official full symbol | tropomyosin 3 |
| Gene type | protein-coding |
| Also known as | CAPM1, CFTD, HEL-189, HEL-S-82p, NEM1, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TM5, TPM3nu, TPMsk3, TRK, hscp30 |
| Summary | This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TPM3-224 | ENST00000651641.1 | 7064 | 285aa | Protein coding | CCDS41403 | P06753-1 | NM_152263.4 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TPM3-223 | ENST00000611659.4 | 3149 | 227aa | Protein coding | CCDS72922 | A0A087WWU8 | - | TSL:3, GENCODE basic, |
| TPM3-212 | ENST00000368533.8 | 3148 | 248aa | Protein coding | CCDS1060 | P06753-2 | - | TSL:1, GENCODE basic, |
| TPM3-206 | ENST00000330188.13 | 2108 | 248aa | Protein coding | CCDS41400 | P06753-5 | - | TSL:1, GENCODE basic, |
| TPM3-205 | ENST00000328159.9 | 1582 | 248aa | Protein coding | CCDS60275 | P06753-6 | - | TSL:2, GENCODE basic, |
| TPM3-204 | ENST00000323144.12 | 1185 | 247aa | Protein coding | CCDS41401 | P06753-4 | - | TSL:2, GENCODE basic, |
| TPM3-211 | ENST00000368531.6 | 1115 | 247aa | Protein coding | CCDS41402 | P06753-3 | - | TSL:1, GENCODE basic, |
| TPM3-202 | ENST00000302206.9 | 741 | 158aa | Protein coding | CCDS60274 | P06753-7 | - | TSL:3, GENCODE basic, |
| TPM3-201 | ENST00000271850.11 | 1219 | 285aa | Protein coding | - | J3KN67 | - | TSL:5, GENCODE basic, |
| TPM3-210 | ENST00000368530.7 | 1111 | 285aa | Protein coding | - | A0A2R2Y2Q3 | - | TSL:1, GENCODE basic, |
| TPM3-222 | ENST00000515609.1 | 587 | 75aa | Protein coding | - | D6RGJ6 | - | CDS 3' incomplete, TSL:3, |
| TPM3-208 | ENST00000341485.10 | 2241 | 247aa | Nonsense mediated decay | CCDS41401 | P06753-4 | - | TSL:1, |
| TPM3-226 | ENST00000651731.1 | 2796 | 97aa | Nonsense mediated decay | - | A0A494C034 | - | CDS 5' incomplete, |
| TPM3-207 | ENST00000341372.8 | 1933 | 49aa | Nonsense mediated decay | - | Q5VU61 | - | TSL:5, |
| TPM3-219 | ENST00000509409.5 | 1248 | 49aa | Nonsense mediated decay | - | Q5VU61 | - | TSL:2, |
| TPM3-227 | ENST00000651873.1 | 1217 | 87aa | Nonsense mediated decay | - | A0A494C0G0 | - | CDS 5' incomplete, |
| TPM3-225 | ENST00000651644.1 | 810 | 214aa | Nonsense mediated decay | - | A0A494C0P6 | - | - |
| TPM3-220 | ENST00000509601.1 | 543 | 95aa | Nonsense mediated decay | - | D6R904 | - | TSL:4, |
| TPM3-203 | ENST00000312970.13 | 1968 | No protein | Processed transcript | - | - | - | TSL:5, |
| TPM3-209 | ENST00000368527.7 | 569 | No protein | Processed transcript | - | - | - | TSL:4, |
| TPM3-214 | ENST00000466010.1 | 461 | No protein | Processed transcript | - | - | - | TSL:2, |
| TPM3-215 | ENST00000469717.5 | 4041 | No protein | Retained intron | - | - | - | TSL:2, |
| TPM3-213 | ENST00000368545.7 | 2147 | No protein | Retained intron | - | - | - | TSL:1, |
| TPM3-217 | ENST00000504663.1 | 870 | No protein | Retained intron | - | - | - | TSL:2, |
| TPM3-216 | ENST00000473036.2 | 746 | No protein | Retained intron | - | - | - | TSL:2, |
| TPM3-218 | ENST00000505010.5 | 540 | No protein | Retained intron | - | - | - | TSL:2, |
| TPM3-221 | ENST00000513769.5 | 539 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TPM3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TPM3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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