CRISPR-U™ Gene Knockout Cell Line Strategy

TPM3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TPM3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TPM3
Gene id 7170
Organism Homo sapiens
Official full symbol tropomyosin 3
Gene type protein-coding
Also known as CAPM1, CFTD, HEL-189, HEL-S-82p, NEM1, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TM5, TPM3nu, TPMsk3, TRK, hscp30
Summary This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TPM3-224 ENST00000651641.1 7064 285aa Protein coding CCDS41403 P06753-1 NM_152263.4 GENCODE basic, APPRIS P1, MANE Select v0.92,
TPM3-223 ENST00000611659.4 3149 227aa Protein coding CCDS72922 A0A087WWU8 - TSL:3, GENCODE basic,
TPM3-212 ENST00000368533.8 3148 248aa Protein coding CCDS1060 P06753-2 - TSL:1, GENCODE basic,
TPM3-206 ENST00000330188.13 2108 248aa Protein coding CCDS41400 P06753-5 - TSL:1, GENCODE basic,
TPM3-205 ENST00000328159.9 1582 248aa Protein coding CCDS60275 P06753-6 - TSL:2, GENCODE basic,
TPM3-204 ENST00000323144.12 1185 247aa Protein coding CCDS41401 P06753-4 - TSL:2, GENCODE basic,
TPM3-211 ENST00000368531.6 1115 247aa Protein coding CCDS41402 P06753-3 - TSL:1, GENCODE basic,
TPM3-202 ENST00000302206.9 741 158aa Protein coding CCDS60274 P06753-7 - TSL:3, GENCODE basic,
TPM3-201 ENST00000271850.11 1219 285aa Protein coding - J3KN67 - TSL:5, GENCODE basic,
TPM3-210 ENST00000368530.7 1111 285aa Protein coding - A0A2R2Y2Q3 - TSL:1, GENCODE basic,
TPM3-222 ENST00000515609.1 587 75aa Protein coding - D6RGJ6 - CDS 3' incomplete, TSL:3,
TPM3-208 ENST00000341485.10 2241 247aa Nonsense mediated decay CCDS41401 P06753-4 - TSL:1,
TPM3-226 ENST00000651731.1 2796 97aa Nonsense mediated decay - A0A494C034 - CDS 5' incomplete,
TPM3-207 ENST00000341372.8 1933 49aa Nonsense mediated decay - Q5VU61 - TSL:5,
TPM3-219 ENST00000509409.5 1248 49aa Nonsense mediated decay - Q5VU61 - TSL:2,
TPM3-227 ENST00000651873.1 1217 87aa Nonsense mediated decay - A0A494C0G0 - CDS 5' incomplete,
TPM3-225 ENST00000651644.1 810 214aa Nonsense mediated decay - A0A494C0P6 - -
TPM3-220 ENST00000509601.1 543 95aa Nonsense mediated decay - D6R904 - TSL:4,
TPM3-203 ENST00000312970.13 1968 No protein Processed transcript - - - TSL:5,
TPM3-209 ENST00000368527.7 569 No protein Processed transcript - - - TSL:4,
TPM3-214 ENST00000466010.1 461 No protein Processed transcript - - - TSL:2,
TPM3-215 ENST00000469717.5 4041 No protein Retained intron - - - TSL:2,
TPM3-213 ENST00000368545.7 2147 No protein Retained intron - - - TSL:1,
TPM3-217 ENST00000504663.1 870 No protein Retained intron - - - TSL:2,
TPM3-216 ENST00000473036.2 746 No protein Retained intron - - - TSL:2,
TPM3-218 ENST00000505010.5 540 No protein Retained intron - - - TSL:2,
TPM3-221 ENST00000513769.5 539 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TPM3
This KO Strategy loading
Red Cotton™ Notes Gene TPM3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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