CRISPR-U™ Gene Knockout Cell Line Strategy

TOP2B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TOP2B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TOP2B
Gene id 7155
Organism Homo sapiens
Official full symbol DNA topoisomerase II beta
Gene type protein-coding
Also known as TOPIIB, top2beta
Summary This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 3
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TOP2B-204 ENST00000435706.6 5389 1621aa Protein coding CCDS46776 Q02880-2 - TSL:1, GENCODE basic, APPRIS P2,
TOP2B-201 ENST00000264331.8 5358 1626aa Protein coding CCDS82746 Q02880-1 - TSL:5, GENCODE basic, APPRIS ALT2,
TOP2B-203 ENST00000424225.1 3525 1150aa Protein coding - E9PCY5 - CDS 3' incomplete, TSL:1,
TOP2B-202 ENST00000413971.5 2049 70aa Nonsense mediated decay - H7BZ82 - CDS 5' incomplete, TSL:5,
TOP2B-206 ENST00000475717.1 540 No protein Processed transcript - - - TSL:4,
TOP2B-207 ENST00000491510.1 584 No protein Retained intron - - - TSL:4,
TOP2B-205 ENST00000470132.1 451 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TOP2B
This KO Strategy loading
Red Cotton™ Notes Gene TOP2B had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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