CRISPR-U™ Gene Knockout Cell Line
Strategy
TOP2B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TOP2B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TOP2B |
| Gene id | 7155 |
| Organism | Homo sapiens |
| Official full symbol | DNA topoisomerase II beta |
| Gene type | protein-coding |
| Also known as | TOPIIB, top2beta |
| Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TOP2B-204 | ENST00000435706.6 | 5389 | 1621aa | Protein coding | CCDS46776 | Q02880-2 | - | TSL:1, GENCODE basic, APPRIS P2, |
| TOP2B-201 | ENST00000264331.8 | 5358 | 1626aa | Protein coding | CCDS82746 | Q02880-1 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| TOP2B-203 | ENST00000424225.1 | 3525 | 1150aa | Protein coding | - | E9PCY5 | - | CDS 3' incomplete, TSL:1, |
| TOP2B-202 | ENST00000413971.5 | 2049 | 70aa | Nonsense mediated decay | - | H7BZ82 | - | CDS 5' incomplete, TSL:5, |
| TOP2B-206 | ENST00000475717.1 | 540 | No protein | Processed transcript | - | - | - | TSL:4, |
| TOP2B-207 | ENST00000491510.1 | 584 | No protein | Retained intron | - | - | - | TSL:4, |
| TOP2B-205 | ENST00000470132.1 | 451 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TOP2B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TOP2B had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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