CRISPR-U™ Gene Knockout Cell Line
Strategy
TNFAIP3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TNFAIP3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TNFAIP3 |
| Gene id | 7128 |
| Organism | Homo sapiens |
| Official full symbol | TNF alpha induced protein 3 |
| Gene type | protein-coding |
| Also known as | A20, AISBL, OTUD7C, TNFA1P2 |
| Summary | This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TNFAIP3-206 | ENST00000612899.5 | 4665 | 790aa | Protein coding | CCDS5187 | P21580 | NM_001270508.2 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TNFAIP3-201 | ENST00000237289.8 | 4432 | 790aa | Protein coding | CCDS5187 | P21580 | - | TSL:1, GENCODE basic, APPRIS P1, |
| TNFAIP3-208 | ENST00000615468.4 | 3818 | 214aa | Protein coding | - | A0A087WVN3 | - | TSL:5, GENCODE basic, |
| TNFAIP3-210 | ENST00000620204.3 | 3817 | 669aa | Protein coding | - | D3TTY5 | - | TSL:5, GENCODE basic, |
| TNFAIP3-209 | ENST00000619035.4 | 2267 | 749aa | Protein coding | - | A0A087WU80 | - | TSL:5, GENCODE basic, |
| TNFAIP3-211 | ENST00000621150.3 | 1613 | 531aa | Protein coding | - | A0A087X1N1 | - | TSL:5, GENCODE basic, |
| TNFAIP3-207 | ENST00000614035.4 | 1430 | 470aa | Protein coding | - | A0A087WXL5 | - | TSL:5, GENCODE basic, |
| TNFAIP3-202 | ENST00000420009.5 | 698 | 162aa | Protein coding | - | Q5VXQ8 | - | CDS 3' incomplete, TSL:3, |
| TNFAIP3-204 | ENST00000433680.1 | 465 | 98aa | Protein coding | - | Q5VXQ9 | - | CDS 3' incomplete, TSL:3, |
| TNFAIP3-203 | ENST00000421450.1 | 421 | 25aa | Protein coding | - | Q5VXR0 | - | CDS 3' incomplete, TSL:2, |
| TNFAIP3-205 | ENST00000485192.1 | 604 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TNFAIP3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TNFAIP3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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