CRISPR-U™ Gene Knockout Cell Line Strategy

TNFAIP3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TNFAIP3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TNFAIP3
Gene id 7128
Organism Homo sapiens
Official full symbol TNF alpha induced protein 3
Gene type protein-coding
Also known as A20, AISBL, OTUD7C, TNFA1P2
Summary This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TNFAIP3-206 ENST00000612899.5 4665 790aa Protein coding CCDS5187 P21580 NM_001270508.2 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
TNFAIP3-201 ENST00000237289.8 4432 790aa Protein coding CCDS5187 P21580 - TSL:1, GENCODE basic, APPRIS P1,
TNFAIP3-208 ENST00000615468.4 3818 214aa Protein coding - A0A087WVN3 - TSL:5, GENCODE basic,
TNFAIP3-210 ENST00000620204.3 3817 669aa Protein coding - D3TTY5 - TSL:5, GENCODE basic,
TNFAIP3-209 ENST00000619035.4 2267 749aa Protein coding - A0A087WU80 - TSL:5, GENCODE basic,
TNFAIP3-211 ENST00000621150.3 1613 531aa Protein coding - A0A087X1N1 - TSL:5, GENCODE basic,
TNFAIP3-207 ENST00000614035.4 1430 470aa Protein coding - A0A087WXL5 - TSL:5, GENCODE basic,
TNFAIP3-202 ENST00000420009.5 698 162aa Protein coding - Q5VXQ8 - CDS 3' incomplete, TSL:3,
TNFAIP3-204 ENST00000433680.1 465 98aa Protein coding - Q5VXQ9 - CDS 3' incomplete, TSL:3,
TNFAIP3-203 ENST00000421450.1 421 25aa Protein coding - Q5VXR0 - CDS 3' incomplete, TSL:2,
TNFAIP3-205 ENST00000485192.1 604 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TNFAIP3
This KO Strategy loading
Red Cotton™ Notes Gene TNFAIP3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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