CRISPR-U™ Gene Knockout Cell Line
Strategy
TIMP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TIMP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TIMP1 |
| Gene id | 7076 |
| Organism | Homo sapiens |
| Official full symbol | TIMP metallopeptidase inhibitor 1 |
| Gene type | protein-coding |
| Also known as | CLGI, EPA, EPO, HCI, TIMP, TIMP-1 |
| Summary | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TIMP1-201 | ENST00000218388.9 | 769 | 207aa | Protein coding | CCDS14281 | P01033 Q6FGX5 | NM_003254.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TIMP1-205 | ENST00000456754.6 | 1095 | 136aa | Protein coding | - | Q5H9B5 | - | TSL:1, GENCODE basic, |
| TIMP1-202 | ENST00000377017.5 | 626 | 143aa | Protein coding | - | Q5H9A7 | - | TSL:2, GENCODE basic, |
| TIMP1-204 | ENST00000445623.1 | 595 | 165aa | Protein coding | - | H0Y789 | - | CDS 5' incomplete, TSL:2, |
| TIMP1-203 | ENST00000441738.1 | 341 | 95aa | Protein coding | - | Q5H9B4 | - | CDS 3' incomplete, TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TIMP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TIMP1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: