CRISPR-U™ Gene Knockout Cell Line Strategy

TIMP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TIMP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TIMP1
Gene id 7076
Organism Homo sapiens
Official full symbol TIMP metallopeptidase inhibitor 1
Gene type protein-coding
Also known as CLGI, EPA, EPO, HCI, TIMP, TIMP-1
Summary This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
Genomic regions Chromosome X
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TIMP1-201 ENST00000218388.9 769 207aa Protein coding CCDS14281 P01033 Q6FGX5 NM_003254.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TIMP1-205 ENST00000456754.6 1095 136aa Protein coding - Q5H9B5 - TSL:1, GENCODE basic,
TIMP1-202 ENST00000377017.5 626 143aa Protein coding - Q5H9A7 - TSL:2, GENCODE basic,
TIMP1-204 ENST00000445623.1 595 165aa Protein coding - H0Y789 - CDS 5' incomplete, TSL:2,
TIMP1-203 ENST00000441738.1 341 95aa Protein coding - Q5H9B4 - CDS 3' incomplete, TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TIMP1
This KO Strategy loading
Red Cotton™ Notes Gene TIMP1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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