CRISPR-U™ Gene Knockout Cell Line Strategy

TIAL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TIAL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TIAL1
Gene id 7073
Organism Homo sapiens
Official full symbol TIA1 cytotoxic granule associated RNA binding protein like 1
Gene type protein-coding
Also known as TCBP, TIAR
Summary The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing.
Genomic regions Chromosome 10
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TIAL1-203 ENST00000369093.6 5072 392aa Protein coding CCDS31295 Q01085-2 - TSL:2, GENCODE basic,
TIAL1-205 ENST00000436547.7 3827 375aa Protein coding CCDS7613 Q01085-1 NM_003252.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TIAL1-204 ENST00000412524.5 515 132aa Protein coding - E7ETJ9 - CDS 3' incomplete, TSL:5,
TIAL1-201 ENST00000369086.1 490 85aa Protein coding - A6NKZ9 - TSL:2,
TIAL1-213 ENST00000497671.5 2321 135aa Nonsense mediated decay - E7ETC0 - TSL:1,
TIAL1-210 ENST00000479729.1 485 28aa Nonsense mediated decay - A0A087WX93 - TSL:3,
TIAL1-207 ENST00000463089.6 907 No protein Processed transcript - - - TSL:5,
TIAL1-208 ENST00000470635.1 336 No protein Processed transcript - - - TSL:3,
TIAL1-206 ENST00000462373.1 260 No protein Processed transcript - - - TSL:3,
TIAL1-211 ENST00000489822.5 2222 No protein Retained intron - - - TSL:2,
TIAL1-209 ENST00000470781.5 1727 No protein Retained intron - - - TSL:2,
TIAL1-202 ENST00000369087.5 1566 No protein Retained intron - - - TSL:2,
TIAL1-212 ENST00000495821.1 372 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TIAL1
This KO Strategy loading
Red Cotton™ Notes Gene TIAL1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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