CRISPR-U™ Gene Knockout Cell Line
Strategy
TGM2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TGM2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TGM2 |
| Gene id | 7052 |
| Organism | Homo sapiens |
| Official full symbol | transglutaminase 2 |
| Gene type | protein-coding |
| Also known as | TG(C), TGC |
| Summary | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TGM2-201 | ENST00000361475.7 | 4970 | 687aa | Protein coding | CCDS13302 | P21980-1 | NM_004613.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TGM2-202 | ENST00000373403.7 | 1018 | 280aa | Protein coding | - | A2A299 | - | CDS 3' incomplete, TSL:3, |
| TGM2-203 | ENST00000453095.1 | 693 | 159aa | Protein coding | - | A2A2A0 | - | CDS 3' incomplete, TSL:4, |
| TGM2-205 | ENST00000469269.1 | 3467 | No protein | Retained intron | - | - | - | TSL:2, |
| TGM2-206 | ENST00000474777.1 | 2287 | No protein | Retained intron | - | - | - | TSL:1, |
| TGM2-204 | ENST00000468262.5 | 1862 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TGM2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TGM2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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