CRISPR-U™ Gene Knockout Cell Line Strategy

TGM2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TGM2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TGM2
Gene id 7052
Organism Homo sapiens
Official full symbol transglutaminase 2
Gene type protein-coding
Also known as TG(C), TGC
Summary Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 20
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TGM2-201 ENST00000361475.7 4970 687aa Protein coding CCDS13302 P21980-1 NM_004613.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TGM2-202 ENST00000373403.7 1018 280aa Protein coding - A2A299 - CDS 3' incomplete, TSL:3,
TGM2-203 ENST00000453095.1 693 159aa Protein coding - A2A2A0 - CDS 3' incomplete, TSL:4,
TGM2-205 ENST00000469269.1 3467 No protein Retained intron - - - TSL:2,
TGM2-206 ENST00000474777.1 2287 No protein Retained intron - - - TSL:1,
TGM2-204 ENST00000468262.5 1862 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TGM2
This KO Strategy loading
Red Cotton™ Notes Gene TGM2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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