CRISPR-U™ Gene Knockout Cell Line Strategy

TGFB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TGFB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TGFB1
Gene id 7040
Organism Homo sapiens
Official full symbol transforming growth factor beta 1
Gene type protein-coding
Also known as CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta
Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TGFB1-201 ENST00000221930.6 2780 390aa Protein coding CCDS33031 A0A499FJK2 NM_000660.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TGFB1-204 ENST00000600196.2 1250 370aa Protein coding - - - TSL:5, GENCODE basic,
TGFB1-205 ENST00000677934.1 1084 306aa Protein coding - - - GENCODE basic,
TGFB1-203 ENST00000598758.5 559 No protein Processed transcript - - - TSL:5,
TGFB1-202 ENST00000597453.1 392 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TGFB1
This KO Strategy loading
Red Cotton™ Notes Gene TGFB1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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