CRISPR-U™ Gene Knockout Cell Line
Strategy
TEAD4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TEAD4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TEAD4 |
| Gene id | 7004 |
| Organism | Homo sapiens |
| Official full symbol | TEA domain transcription factor 4 |
| Gene type | protein-coding |
| Also known as | EFTR-2, RTEF1, TCF13L1, TEF-3, TEF3, TEFR-1, hRTEF-1B |
| Summary | This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TEAD4-202 | ENST00000359864.8 | 1710 | 434aa | Protein coding | CCDS31729 | Q53GI4 | NM_003213.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TEAD4-201 | ENST00000358409.7 | 1637 | 391aa | Protein coding | CCDS31730 | A0A0A0MRF3 | - | TSL:1, GENCODE basic, |
| TEAD4-203 | ENST00000397122.6 | 1396 | 305aa | Protein coding | CCDS41737 | Q15561-2 | - | TSL:1, GENCODE basic, |
| TEAD4-207 | ENST00000543035.6 | 801 | 175aa | Protein coding | - | H0YGS2 | - | CDS 3' incomplete, TSL:5, |
| TEAD4-206 | ENST00000540314.2 | 578 | 22aa | Protein coding | - | H0YFK0 | - | CDS 3' incomplete, TSL:4, |
| TEAD4-205 | ENST00000536826.2 | 570 | 72aa | Protein coding | - | H0YFF9 | - | CDS 3' incomplete, TSL:4, |
| TEAD4-208 | ENST00000544666.1 | 567 | 189aa | Protein coding | - | H0YG62 | - | CDS 5' and 3' incomplete, TSL:5, |
| TEAD4-204 | ENST00000443986.3 | 641 | 110aa | Nonsense mediated decay | - | H0Y7B0 | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TEAD4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TEAD4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: