CRISPR-U™ Gene Knockout Cell Line
Strategy
DYNLT3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DYNLT3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DYNLT3 |
| Gene id | 6990 |
| Organism | Homo sapiens |
| Official full symbol | dynein light chain Tctex-type 3 |
| Gene type | protein-coding |
| Also known as | RP3, TCTE1L, TCTEX1L |
| Summary | This gene encodes a member of a subclass of dynein light chains. The encoded protein homodimerizes and forms the light chain component of the cytoplasmic dynein motor protein complex. This protein may be important for binding dynein to specific cargos including the spindle checkpoint protein BUB3. This protein may also function independently of dynein as a transcriptional modulator. Pseudogenes of this gene are found on chromosomes 2 and 20. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DYNLT3-201 | ENST00000378578.9 | 2151 | 116aa | Protein coding | CCDS14243 | P51808 | NM_006520.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DYNLT3-202 | ENST00000378581.7 | 920 | 152aa | Protein coding | - | A6NGJ0 | - | TSL:2, GENCODE basic, |
| DYNLT3-203 | ENST00000432389.2 | 719 | 122aa | Protein coding | - | F2Z328 | - | TSL:5, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DYNLT3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DYNLT3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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