CRISPR-U™ Gene Knockout Cell Line Strategy

SYP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SYP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SYP
Gene id 6855
Organism Homo sapiens
Official full symbol synaptophysin
Gene type protein-coding
Also known as MRX96, MRXSYP
Summary This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability.
Genomic regions Chromosome X
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SYP-201 ENST00000263233.9 2421 313aa Protein coding CCDS14321 P08247-1 NM_003179.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SYP-207 ENST00000479808.5 1850 313aa Protein coding CCDS14321 P08247-1 - TSL:1, GENCODE basic, APPRIS P1,
SYP-205 ENST00000472598.5 761 203aa Protein coding - H7C4W3 - CDS 5' incomplete, TSL:3,
SYP-202 ENST00000376303.6 858 40aa Nonsense mediated decay - G5E9A2 - TSL:2,
SYP-204 ENST00000469389.5 581 118aa Nonsense mediated decay - F2Z3E1 - TSL:4,
SYP-203 ENST00000466635.1 513 118aa Nonsense mediated decay - F2Z3E1 - TSL:4,
SYP-208 ENST00000494396.5 2062 No protein Retained intron - - - TSL:2,
SYP-206 ENST00000472737.1 574 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SYP
This KO Strategy loading
Red Cotton™ Notes Gene SYP had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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