CRISPR-U™ Gene Knockout Cell Line
Strategy
SYP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SYP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SYP |
| Gene id | 6855 |
| Organism | Homo sapiens |
| Official full symbol | synaptophysin |
| Gene type | protein-coding |
| Also known as | MRX96, MRXSYP |
| Summary | This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SYP-201 | ENST00000263233.9 | 2421 | 313aa | Protein coding | CCDS14321 | P08247-1 | NM_003179.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SYP-207 | ENST00000479808.5 | 1850 | 313aa | Protein coding | CCDS14321 | P08247-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| SYP-205 | ENST00000472598.5 | 761 | 203aa | Protein coding | - | H7C4W3 | - | CDS 5' incomplete, TSL:3, |
| SYP-202 | ENST00000376303.6 | 858 | 40aa | Nonsense mediated decay | - | G5E9A2 | - | TSL:2, |
| SYP-204 | ENST00000469389.5 | 581 | 118aa | Nonsense mediated decay | - | F2Z3E1 | - | TSL:4, |
| SYP-203 | ENST00000466635.1 | 513 | 118aa | Nonsense mediated decay | - | F2Z3E1 | - | TSL:4, |
| SYP-208 | ENST00000494396.5 | 2062 | No protein | Retained intron | - | - | - | TSL:2, |
| SYP-206 | ENST00000472737.1 | 574 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SYP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SYP had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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