CRISPR-U™ Gene Knockout Cell Line
Strategy
BST2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BST2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BST2 |
| Gene id | 684 |
| Organism | Homo sapiens |
| Official full symbol | bone marrow stromal cell antigen 2 |
| Gene type | protein-coding |
| Also known as | CD317, TETHERIN |
| Summary | Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BST2-201 | ENST00000252593.7 | 1001 | 180aa | Protein coding | CCDS12358 | Q10589-1 | NM_004335.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BST2-202 | ENST00000527220.2 | 879 | 100aa | Nonsense mediated decay | - | - | - | TSL:2, |
| BST2-203 | ENST00000533098.5 | 459 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BST2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BST2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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