CRISPR-U™ Gene Knockout Cell Line
Strategy
STK3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human STK3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | STK3 |
| Gene id | 6788 |
| Organism | Homo sapiens |
| Official full symbol | serine/threonine kinase 3 |
| Gene type | protein-coding |
| Also known as | KRS1, MST2 |
| Summary | This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| STK3-217 | ENST00000523601.5 | 3107 | 519aa | Protein coding | CCDS59108 | Q13188-2 | - | TSL:2, GENCODE basic, |
| STK3-201 | ENST00000419617.7 | 2833 | 491aa | Protein coding | CCDS47900 | Q13188-1 | NM_006281.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| STK3-219 | ENST00000617590.1 | 2485 | 380aa | Protein coding | CCDS75774 | A0A087WZ06 | - | TSL:5, GENCODE basic, |
| STK3-204 | ENST00000518165.5 | 817 | 228aa | Protein coding | - | E5RFQ9 | - | CDS 3' incomplete, TSL:1, |
| STK3-208 | ENST00000519420.1 | 531 | 1aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:4, |
| STK3-202 | ENST00000424861.6 | 2065 | 168aa | Nonsense mediated decay | - | Q8NBU1 | - | TSL:2, |
| STK3-212 | ENST00000521649.5 | 574 | 82aa | Nonsense mediated decay | - | E5RIM6 | - | TSL:4, |
| STK3-213 | ENST00000521768.5 | 1016 | No protein | Processed transcript | - | - | - | TSL:5, |
| STK3-220 | ENST00000649151.1 | 989 | No protein | Processed transcript | - | - | - | - |
| STK3-205 | ENST00000518582.5 | 725 | No protein | Processed transcript | - | - | - | TSL:3, |
| STK3-203 | ENST00000517832.1 | 594 | No protein | Processed transcript | - | - | - | TSL:3, |
| STK3-216 | ENST00000523567.1 | 587 | No protein | Processed transcript | - | - | - | TSL:3, |
| STK3-215 | ENST00000523159.5 | 537 | No protein | Processed transcript | - | - | - | TSL:3, |
| STK3-211 | ENST00000520653.1 | 531 | No protein | Processed transcript | - | - | - | TSL:4, |
| STK3-214 | ENST00000522924.1 | 510 | No protein | Processed transcript | - | - | - | TSL:3, |
| STK3-206 | ENST00000518627.5 | 468 | No protein | Processed transcript | - | - | - | TSL:3, |
| STK3-207 | ENST00000518704.1 | 435 | No protein | Processed transcript | - | - | - | TSL:3, |
| STK3-218 | ENST00000523960.1 | 5333 | No protein | Retained intron | - | - | - | TSL:1, |
| STK3-210 | ENST00000520440.1 | 697 | No protein | Retained intron | - | - | - | TSL:2, |
| STK3-209 | ENST00000520053.1 | 577 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | STK3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene STK3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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