CRISPR-U™ Gene Knockout Cell Line Strategy

STK3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human STK3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol STK3
Gene id 6788
Organism Homo sapiens
Official full symbol serine/threonine kinase 3
Gene type protein-coding
Also known as KRS1, MST2
Summary This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 8
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
STK3-217 ENST00000523601.5 3107 519aa Protein coding CCDS59108 Q13188-2 - TSL:2, GENCODE basic,
STK3-201 ENST00000419617.7 2833 491aa Protein coding CCDS47900 Q13188-1 NM_006281.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
STK3-219 ENST00000617590.1 2485 380aa Protein coding CCDS75774 A0A087WZ06 - TSL:5, GENCODE basic,
STK3-204 ENST00000518165.5 817 228aa Protein coding - E5RFQ9 - CDS 3' incomplete, TSL:1,
STK3-208 ENST00000519420.1 531 1aa Protein coding - - - CDS 3' incomplete, TSL:4,
STK3-202 ENST00000424861.6 2065 168aa Nonsense mediated decay - Q8NBU1 - TSL:2,
STK3-212 ENST00000521649.5 574 82aa Nonsense mediated decay - E5RIM6 - TSL:4,
STK3-213 ENST00000521768.5 1016 No protein Processed transcript - - - TSL:5,
STK3-220 ENST00000649151.1 989 No protein Processed transcript - - - -
STK3-205 ENST00000518582.5 725 No protein Processed transcript - - - TSL:3,
STK3-203 ENST00000517832.1 594 No protein Processed transcript - - - TSL:3,
STK3-216 ENST00000523567.1 587 No protein Processed transcript - - - TSL:3,
STK3-215 ENST00000523159.5 537 No protein Processed transcript - - - TSL:3,
STK3-211 ENST00000520653.1 531 No protein Processed transcript - - - TSL:4,
STK3-214 ENST00000522924.1 510 No protein Processed transcript - - - TSL:3,
STK3-206 ENST00000518627.5 468 No protein Processed transcript - - - TSL:3,
STK3-207 ENST00000518704.1 435 No protein Processed transcript - - - TSL:3,
STK3-218 ENST00000523960.1 5333 No protein Retained intron - - - TSL:1,
STK3-210 ENST00000520440.1 697 No protein Retained intron - - - TSL:2,
STK3-209 ENST00000520053.1 577 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene STK3
This KO Strategy loading
Red Cotton™ Notes Gene STK3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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