CRISPR-U™ Gene Knockout Cell Line Strategy

STAT5B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human STAT5B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol STAT5B
Gene id 6777
Organism Homo sapiens
Official full symbol signal transducer and activator of transcription 5B
Gene type protein-coding
Also known as STAT5
Summary The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.
Genomic regions Chromosome 17
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
STAT5B-201 ENST00000293328.8 5079 787aa Protein coding CCDS11423 P51692 NM_012448.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
STAT5B-202 ENST00000415845.1 559 156aa Protein coding - C9J4I3 - CDS 3' incomplete, TSL:4,
STAT5B-203 ENST00000468312.1 1799 No protein Retained intron - - - TSL:1,
STAT5B-206 ENST00000481517.1 999 No protein Retained intron - - - TSL:4,
STAT5B-204 ENST00000468496.5 897 No protein Retained intron - - - TSL:5,
STAT5B-207 ENST00000498674.1 542 No protein Retained intron - - - TSL:4,
STAT5B-205 ENST00000481253.2 491 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene STAT5B
This KO Strategy loading
Red Cotton™ Notes Gene STAT5B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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