CRISPR-U™ Gene Knockout Cell Line Strategy

STAT3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human STAT3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol STAT3
Gene id 6774
Organism Homo sapiens
Official full symbol signal transducer and activator of transcription 3
Gene type protein-coding
Also known as ADMIO, ADMIO1, APRF, HIES
Summary The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Genomic regions Chromosome 17
Strategy Summary
This gene has 28 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
STAT3-244 ENST00000678906.1 4971 770aa Protein coding CCDS32656 - - GENCODE basic, APPRIS ALT1,
STAT3-201 ENST00000264657.10 4921 770aa Protein coding CCDS32656 P40763-1 NM_139276.3 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
STAT3-231 ENST00000678044.1 4912 770aa Protein coding CCDS32656 - - GENCODE basic, APPRIS ALT1,
STAT3-246 ENST00000678960.1 4912 770aa Protein coding CCDS32656 - - GENCODE basic, APPRIS ALT1,
STAT3-247 ENST00000679014.1 4890 769aa Protein coding CCDS32657 - - GENCODE basic, APPRIS ALT1,
STAT3-227 ENST00000677723.1 4834 769aa Protein coding CCDS32657 - - GENCODE basic, APPRIS ALT1,
STAT3-216 ENST00000677030.1 4812 722aa Protein coding CCDS59288 - - GENCODE basic,
STAT3-242 ENST00000678827.1 4800 722aa Protein coding CCDS59288 - - GENCODE basic,
STAT3-211 ENST00000585517.5 3319 722aa Protein coding CCDS59288 P40763-3 - TSL:5, GENCODE basic,
STAT3-212 ENST00000588969.5 2772 770aa Protein coding CCDS32656 P40763-1 - TSL:1, GENCODE basic, APPRIS ALT1,
STAT3-203 ENST00000404395.3 2633 769aa Protein coding CCDS32657 P40763-2 - TSL:1, GENCODE basic, APPRIS P4,
STAT3-222 ENST00000677421.1 4911 802aa Protein coding - - - GENCODE basic,
STAT3-241 ENST00000678792.1 4842 775aa Protein coding - - - GENCODE basic,
STAT3-245 ENST00000678913.1 4839 775aa Protein coding - - - GENCODE basic,
STAT3-230 ENST00000678043.1 4837 763aa Protein coding - - - GENCODE basic,
STAT3-223 ENST00000677442.1 4829 761aa Protein coding - - - GENCODE basic,
STAT3-249 ENST00000679185.1 4782 721aa Protein coding - - - GENCODE basic,
STAT3-243 ENST00000678905.1 4768 726aa Protein coding - - - GENCODE basic,
STAT3-248 ENST00000679166.1 4748 742aa Protein coding - - - GENCODE basic,
STAT3-232 ENST00000678048.1 4740 742aa Protein coding - - - GENCODE basic,
STAT3-237 ENST00000678572.1 4739 727aa Protein coding - - - GENCODE basic,
STAT3-224 ENST00000677479.1 4731 721aa Protein coding - - - GENCODE basic,
STAT3-239 ENST00000678674.1 4703 737aa Protein coding - - - GENCODE basic,
STAT3-217 ENST00000677152.1 3395 765aa Protein coding - - - GENCODE basic,
STAT3-226 ENST00000677603.1 3314 738aa Protein coding - - - GENCODE basic,
STAT3-236 ENST00000678535.1 3288 738aa Protein coding - - - GENCODE basic,
STAT3-215 ENST00000677002.1 3180 760aa Protein coding - - - GENCODE basic,
STAT3-202 ENST00000389272.7 2615 672aa Protein coding - G8JLH9 - TSL:2, GENCODE basic,
STAT3-234 ENST00000678445.1 4944 575aa Nonsense mediated decay - - - -
STAT3-229 ENST00000677820.1 4879 575aa Nonsense mediated decay - - - -
STAT3-214 ENST00000676636.1 4822 575aa Nonsense mediated decay - - - -
STAT3-219 ENST00000677271.1 4489 458aa Nonsense mediated decay - - - -
STAT3-220 ENST00000677308.1 3374 575aa Nonsense mediated decay - - - -
STAT3-221 ENST00000677346.1 1486 No protein Processed transcript - - - -
STAT3-213 ENST00000590776.1 1483 No protein Processed transcript - - - TSL:NA,
STAT3-208 ENST00000491272.2 1327 No protein Processed transcript - - - TSL:2,
STAT3-225 ENST00000677500.1 1277 No protein Processed transcript - - - -
STAT3-205 ENST00000462286.3 1248 No protein Processed transcript - - - TSL:NA,
STAT3-240 ENST00000678764.1 5054 No protein Retained intron - - - -
STAT3-250 ENST00000679231.1 4880 No protein Retained intron - - - -
STAT3-228 ENST00000677763.1 3027 No protein Retained intron - - - -
STAT3-238 ENST00000678659.1 2866 No protein Retained intron - - - -
STAT3-218 ENST00000677270.1 2680 No protein Retained intron - - - -
STAT3-235 ENST00000678529.1 2118 No protein Retained intron - - - -
STAT3-233 ENST00000678108.1 1927 No protein Retained intron - - - -
STAT3-206 ENST00000471989.5 826 No protein Retained intron - - - TSL:2,
STAT3-204 ENST00000462269.1 698 No protein Retained intron - - - TSL:2,
STAT3-209 ENST00000498330.1 559 No protein Retained intron - - - TSL:2,
STAT3-210 ENST00000585360.1 517 No protein Retained intron - - - TSL:3,
STAT3-207 ENST00000478276.1 186 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene STAT3
This KO Strategy loading
Red Cotton™ Notes Gene STAT3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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