CRISPR-U™ Gene Knockout Cell Line
Strategy
STAT2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human STAT2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | STAT2 |
| Gene id | 6773 |
| Organism | Homo sapiens |
| Official full symbol | signal transducer and activator of transcription 2 |
| Gene type | protein-coding |
| Also known as | IMD44, ISGF-3, P113, PTORCH3, STAT113 |
| Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Mutations in this gene result in Immunodeficiency 44. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| STAT2-201 | ENST00000314128.9 | 4405 | 851aa | Protein coding | CCDS8917 | P52630-3 | NM_005419.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| STAT2-210 | ENST00000557235.5 | 3073 | 847aa | Protein coding | CCDS55836 | P52630-4 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| STAT2-218 | ENST00000651915.1 | 4319 | 818aa | Protein coding | - | A0A494C164 | - | GENCODE basic, |
| STAT2-224 | ENST00000652741.1 | 4740 | 203aa | Nonsense mediated decay | - | B4DHE0 | - | - |
| STAT2-215 | ENST00000651301.1 | 4670 | 199aa | Nonsense mediated decay | - | A0A494C0B5 | - | - |
| STAT2-213 | ENST00000650805.1 | 4465 | 203aa | Nonsense mediated decay | - | B4DHE0 | - | - |
| STAT2-223 | ENST00000652624.1 | 4124 | 261aa | Nonsense mediated decay | - | A0A494C1L3 | - | - |
| STAT2-222 | ENST00000652398.1 | 3131 | 203aa | Nonsense mediated decay | - | B4DHE0 | - | - |
| STAT2-202 | ENST00000418572.7 | 1675 | 460aa | Nonsense mediated decay | - | B4DLC8 | - | TSL:2, |
| STAT2-205 | ENST00000555646.1 | 603 | 65aa | Nonsense mediated decay | - | G3V319 | - | TSL:4, |
| STAT2-207 | ENST00000556539.5 | 3278 | No protein | Processed transcript | - | - | - | TSL:1, |
| STAT2-216 | ENST00000651339.1 | 2050 | No protein | Processed transcript | - | - | - | - |
| STAT2-211 | ENST00000557252.1 | 717 | No protein | Processed transcript | - | - | - | TSL:2, |
| STAT2-208 | ENST00000557156.1 | 539 | No protein | Processed transcript | - | - | - | TSL:4, |
| STAT2-214 | ENST00000651078.1 | 4985 | No protein | Retained intron | - | - | - | - |
| STAT2-221 | ENST00000652091.1 | 4850 | No protein | Retained intron | - | - | - | - |
| STAT2-219 | ENST00000651934.1 | 4772 | No protein | Retained intron | - | - | - | - |
| STAT2-217 | ENST00000651805.1 | 4721 | No protein | Retained intron | - | - | - | - |
| STAT2-206 | ENST00000556140.6 | 4419 | No protein | Retained intron | - | - | - | TSL:2, |
| STAT2-220 | ENST00000651967.1 | 3442 | No protein | Retained intron | - | - | - | - |
| STAT2-212 | ENST00000557417.5 | 1063 | No protein | Retained intron | - | - | - | TSL:2, |
| STAT2-209 | ENST00000557199.1 | 662 | No protein | Retained intron | - | - | - | TSL:4, |
| STAT2-204 | ENST00000555519.5 | 588 | No protein | Retained intron | - | - | - | TSL:5, |
| STAT2-203 | ENST00000555488.1 | 536 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | STAT2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene STAT2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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