CRISPR-U™ Gene Knockout Cell Line Strategy

STAT2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human STAT2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol STAT2
Gene id 6773
Organism Homo sapiens
Official full symbol signal transducer and activator of transcription 2
Gene type protein-coding
Also known as IMD44, ISGF-3, P113, PTORCH3, STAT113
Summary The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Mutations in this gene result in Immunodeficiency 44.
Genomic regions Chromosome 12
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
STAT2-201 ENST00000314128.9 4405 851aa Protein coding CCDS8917 P52630-3 NM_005419.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
STAT2-210 ENST00000557235.5 3073 847aa Protein coding CCDS55836 P52630-4 - TSL:2, GENCODE basic, APPRIS ALT2,
STAT2-218 ENST00000651915.1 4319 818aa Protein coding - A0A494C164 - GENCODE basic,
STAT2-224 ENST00000652741.1 4740 203aa Nonsense mediated decay - B4DHE0 - -
STAT2-215 ENST00000651301.1 4670 199aa Nonsense mediated decay - A0A494C0B5 - -
STAT2-213 ENST00000650805.1 4465 203aa Nonsense mediated decay - B4DHE0 - -
STAT2-223 ENST00000652624.1 4124 261aa Nonsense mediated decay - A0A494C1L3 - -
STAT2-222 ENST00000652398.1 3131 203aa Nonsense mediated decay - B4DHE0 - -
STAT2-202 ENST00000418572.7 1675 460aa Nonsense mediated decay - B4DLC8 - TSL:2,
STAT2-205 ENST00000555646.1 603 65aa Nonsense mediated decay - G3V319 - TSL:4,
STAT2-207 ENST00000556539.5 3278 No protein Processed transcript - - - TSL:1,
STAT2-216 ENST00000651339.1 2050 No protein Processed transcript - - - -
STAT2-211 ENST00000557252.1 717 No protein Processed transcript - - - TSL:2,
STAT2-208 ENST00000557156.1 539 No protein Processed transcript - - - TSL:4,
STAT2-214 ENST00000651078.1 4985 No protein Retained intron - - - -
STAT2-221 ENST00000652091.1 4850 No protein Retained intron - - - -
STAT2-219 ENST00000651934.1 4772 No protein Retained intron - - - -
STAT2-217 ENST00000651805.1 4721 No protein Retained intron - - - -
STAT2-206 ENST00000556140.6 4419 No protein Retained intron - - - TSL:2,
STAT2-220 ENST00000651967.1 3442 No protein Retained intron - - - -
STAT2-212 ENST00000557417.5 1063 No protein Retained intron - - - TSL:2,
STAT2-209 ENST00000557199.1 662 No protein Retained intron - - - TSL:4,
STAT2-204 ENST00000555519.5 588 No protein Retained intron - - - TSL:5,
STAT2-203 ENST00000555488.1 536 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene STAT2
This KO Strategy loading
Red Cotton™ Notes Gene STAT2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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