CRISPR-U™ Gene Knockout Cell Line
Strategy
STAT1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human STAT1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | STAT1 |
| Gene id | 6772 |
| Organism | Homo sapiens |
| Official full symbol | signal transducer and activator of transcription 1 |
| Gene type | protein-coding |
| Also known as | CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 |
| Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 14 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| STAT1-201 | ENST00000361099.8 | 4116 | 750aa | Protein coding | CCDS2309 | P42224-1 | NM_007315.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| STAT1-212 | ENST00000540176.6 | 4020 | 750aa | Protein coding | CCDS2309 | P42224-1 | - | TSL:5, GENCODE basic, APPRIS P2, |
| STAT1-204 | ENST00000409465.5 | 3097 | 750aa | Protein coding | CCDS2309 | P42224-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| STAT1-202 | ENST00000392322.7 | 2716 | 712aa | Protein coding | CCDS42793 | P42224-2 | - | TSL:1, GENCODE basic, |
| STAT1-219 | ENST00000673841.1 | 2625 | 712aa | Protein coding | CCDS42793 | P42224-2 | - | GENCODE basic, |
| STAT1-225 | ENST00000673942.1 | 3937 | 748aa | Protein coding | - | A0A669KB68 | - | GENCODE basic, APPRIS ALT1, |
| STAT1-216 | ENST00000673777.1 | 3875 | 748aa | Protein coding | - | A0A669KB68 | - | GENCODE basic, APPRIS ALT1, |
| STAT1-220 | ENST00000673847.1 | 3559 | 779aa | Protein coding | - | A0A669KBA4 | - | GENCODE basic, |
| STAT1-226 | ENST00000673952.1 | 3385 | 791aa | Protein coding | - | A0A669KB53 | - | GENCODE basic, |
| STAT1-217 | ENST00000673816.1 | 3145 | 748aa | Protein coding | - | A0A669KB56 | - | GENCODE basic, APPRIS ALT1, |
| STAT1-203 | ENST00000392323.6 | 2723 | 714aa | Protein coding | - | J3KPM9 | - | TSL:2, GENCODE basic, |
| STAT1-207 | ENST00000424722.6 | 1430 | 377aa | Protein coding | - | E7EPD2 | - | CDS 3' incomplete, TSL:3, |
| STAT1-210 | ENST00000454414.5 | 572 | 150aa | Protein coding | - | E7ENM1 | - | CDS 3' incomplete, TSL:4, |
| STAT1-208 | ENST00000432058.1 | 567 | 69aa | Protein coding | - | E9PH66 | - | CDS 3' incomplete, TSL:4, |
| STAT1-205 | ENST00000415035.2 | 3167 | 750aa | Nonsense mediated decay | CCDS2309 | P42224-1 | - | TSL:3, |
| STAT1-229 | ENST00000674081.1 | 4134 | 716aa | Nonsense mediated decay | - | A0A669KBI6 | - | - |
| STAT1-214 | ENST00000673734.1 | 3925 | 273aa | Nonsense mediated decay | - | A0A669KB17 | - | - |
| STAT1-221 | ENST00000673858.1 | 3894 | 193aa | Nonsense mediated decay | - | D2KFR9 | - | - |
| STAT1-228 | ENST00000674080.1 | 2870 | 712aa | Nonsense mediated decay | - | P42224-2 | - | - |
| STAT1-230 | ENST00000674153.1 | 2789 | 636aa | Nonsense mediated decay | - | A0A669KB52 | - | - |
| STAT1-209 | ENST00000452281.6 | 2683 | 193aa | Nonsense mediated decay | - | D2KFR9 | - | TSL:1, |
| STAT1-224 | ENST00000673885.1 | 2649 | 572aa | Nonsense mediated decay | - | A0A669KB75 | - | - |
| STAT1-223 | ENST00000673863.1 | 2397 | 26aa | Nonsense mediated decay | - | A0A669KBK4 | - | CDS 5' incomplete, |
| STAT1-206 | ENST00000423282.2 | 2239 | 42aa | Nonsense mediated decay | - | Q67C41 | - | TSL:3, |
| STAT1-215 | ENST00000673762.1 | 2305 | No protein | Processed transcript | - | - | - | - |
| STAT1-218 | ENST00000673832.1 | 2240 | No protein | Processed transcript | - | - | - | - |
| STAT1-227 | ENST00000674028.1 | 2141 | No protein | Processed transcript | - | - | - | - |
| STAT1-213 | ENST00000673638.1 | 2451 | No protein | Retained intron | - | - | - | - |
| STAT1-222 | ENST00000673859.1 | 2076 | No protein | Retained intron | - | - | - | - |
| STAT1-211 | ENST00000464072.1 | 865 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | STAT1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene STAT1 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: