CRISPR-U™ Gene Knockout Cell Line Strategy

STAT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human STAT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol STAT1
Gene id 6772
Organism Homo sapiens
Official full symbol signal transducer and activator of transcription 1
Gene type protein-coding
Also known as CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91
Summary The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.
Genomic regions Chromosome 2
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
STAT1-201 ENST00000361099.8 4116 750aa Protein coding CCDS2309 P42224-1 NM_007315.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
STAT1-212 ENST00000540176.6 4020 750aa Protein coding CCDS2309 P42224-1 - TSL:5, GENCODE basic, APPRIS P2,
STAT1-204 ENST00000409465.5 3097 750aa Protein coding CCDS2309 P42224-1 - TSL:1, GENCODE basic, APPRIS P2,
STAT1-202 ENST00000392322.7 2716 712aa Protein coding CCDS42793 P42224-2 - TSL:1, GENCODE basic,
STAT1-219 ENST00000673841.1 2625 712aa Protein coding CCDS42793 P42224-2 - GENCODE basic,
STAT1-225 ENST00000673942.1 3937 748aa Protein coding - A0A669KB68 - GENCODE basic, APPRIS ALT1,
STAT1-216 ENST00000673777.1 3875 748aa Protein coding - A0A669KB68 - GENCODE basic, APPRIS ALT1,
STAT1-220 ENST00000673847.1 3559 779aa Protein coding - A0A669KBA4 - GENCODE basic,
STAT1-226 ENST00000673952.1 3385 791aa Protein coding - A0A669KB53 - GENCODE basic,
STAT1-217 ENST00000673816.1 3145 748aa Protein coding - A0A669KB56 - GENCODE basic, APPRIS ALT1,
STAT1-203 ENST00000392323.6 2723 714aa Protein coding - J3KPM9 - TSL:2, GENCODE basic,
STAT1-207 ENST00000424722.6 1430 377aa Protein coding - E7EPD2 - CDS 3' incomplete, TSL:3,
STAT1-210 ENST00000454414.5 572 150aa Protein coding - E7ENM1 - CDS 3' incomplete, TSL:4,
STAT1-208 ENST00000432058.1 567 69aa Protein coding - E9PH66 - CDS 3' incomplete, TSL:4,
STAT1-205 ENST00000415035.2 3167 750aa Nonsense mediated decay CCDS2309 P42224-1 - TSL:3,
STAT1-229 ENST00000674081.1 4134 716aa Nonsense mediated decay - A0A669KBI6 - -
STAT1-214 ENST00000673734.1 3925 273aa Nonsense mediated decay - A0A669KB17 - -
STAT1-221 ENST00000673858.1 3894 193aa Nonsense mediated decay - D2KFR9 - -
STAT1-228 ENST00000674080.1 2870 712aa Nonsense mediated decay - P42224-2 - -
STAT1-230 ENST00000674153.1 2789 636aa Nonsense mediated decay - A0A669KB52 - -
STAT1-209 ENST00000452281.6 2683 193aa Nonsense mediated decay - D2KFR9 - TSL:1,
STAT1-224 ENST00000673885.1 2649 572aa Nonsense mediated decay - A0A669KB75 - -
STAT1-223 ENST00000673863.1 2397 26aa Nonsense mediated decay - A0A669KBK4 - CDS 5' incomplete,
STAT1-206 ENST00000423282.2 2239 42aa Nonsense mediated decay - Q67C41 - TSL:3,
STAT1-215 ENST00000673762.1 2305 No protein Processed transcript - - - -
STAT1-218 ENST00000673832.1 2240 No protein Processed transcript - - - -
STAT1-227 ENST00000674028.1 2141 No protein Processed transcript - - - -
STAT1-213 ENST00000673638.1 2451 No protein Retained intron - - - -
STAT1-222 ENST00000673859.1 2076 No protein Retained intron - - - -
STAT1-211 ENST00000464072.1 865 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene STAT1
This KO Strategy loading
Red Cotton™ Notes Gene STAT1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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