CRISPR-U™ Gene Knockout Cell Line Strategy

BRCA2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BRCA2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BRCA2
Gene id 675
Organism Homo sapiens
Official full symbol BRCA2 DNA repair associated
Gene type protein-coding
Summary Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The largest exon in both genes is exon 11, which harbors the most important and frequent mutations in breast cancer patients. The BRCA2 gene was found on chromosome 13q12.3 in human. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.
Genomic regions Chromosome 13
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BRCA2-201 ENST00000380152.8 11954 3418aa Protein coding CCDS9344 P51587 NM_000059.4 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
BRCA2-210 ENST00000680887.1 11880 3418aa Protein coding CCDS9344 - - APPRIS P1,
BRCA2-206 ENST00000544455.6 11854 3418aa Protein coding CCDS9344 P51587 - TSL:1, GENCODE basic, APPRIS P1,
BRCA2-204 ENST00000530893.6 2011 481aa Protein coding - A0A590UJI7 - CDS 3' incomplete, TSL:1,
BRCA2-207 ENST00000614259.2 11763 2649aa Nonsense mediated decay - - - TSL:2,
BRCA2-208 ENST00000665585.1 2598 438aa Nonsense mediated decay - A0A590UJU6 - CDS 5' incomplete,
BRCA2-202 ENST00000470094.1 842 186aa Nonsense mediated decay - H0YE37 - CDS 5' incomplete, TSL:5,
BRCA2-209 ENST00000666593.1 523 58aa Nonsense mediated decay - A0A590UJ24 - CDS 5' incomplete,
BRCA2-203 ENST00000528762.1 495 64aa Nonsense mediated decay - H0YD86 - CDS 5' incomplete, TSL:4,
BRCA2-205 ENST00000533776.1 523 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BRCA2
This KO Strategy loading
Red Cotton™ Notes Gene BRCA2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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