CRISPR-U™ Gene Knockout Cell Line
Strategy
TRIM21 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TRIM21 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TRIM21 |
| Gene id | 6737 |
| Organism | Homo sapiens |
| Official full symbol | tripartite motif containing 21 |
| Gene type | protein-coding |
| Also known as | RNF81, RO52, Ro/SSA, SSA, SSA1 |
| Summary | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TRIM21-201 | ENST00000254436.8 | 1935 | 475aa | Protein coding | CCDS44525 | P19474-1 | NM_003141.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TRIM21-202 | ENST00000533692.1 | 706 | 90aa | Protein coding | - | H0YDP8 | - | CDS 5' incomplete, TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TRIM21 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TRIM21 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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