CRISPR-U™ Gene Knockout Cell Line
Strategy
BRAF Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BRAF Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BRAF |
| Gene id | 673 |
| Organism | Homo sapiens |
| Official full symbol | B-Raf proto-oncogene, serine/threonine kinase |
| Gene type | protein-coding |
| Also known as | B-RAF1, B-raf, BRAF1, NS7, RAFB1 |
| Summary | This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BRAF-204 | ENST00000496384.7 | 9578 | 767aa | Protein coding | CCDS87555 | H7C560 | - | TSL:5, GENCODE basic, APPRIS P3, |
| BRAF-220 | ENST00000646891.1 | 6458 | 766aa | Protein coding | CCDS5863 | P15056 | - | GENCODE basic, APPRIS ALT2, |
| BRAF-215 | ENST00000644969.2 | 9807 | 807aa | Protein coding | - | A0A2R8Y8E0 | NM_001374258.1 | GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| BRAF-201 | ENST00000288602.11 | 2561 | 806aa | Protein coding | - | A0A2U3TZI2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| BRAF-202 | ENST00000469930.2 | 1180 | 172aa | Protein coding | - | A0A2R8YES9 | - | TSL:2, GENCODE basic, |
| BRAF-219 | ENST00000646730.1 | 2829 | 584aa | Nonsense mediated decay | - | A0A2R8YDP5 | - | - |
| BRAF-206 | ENST00000642228.1 | 2574 | 300aa | Nonsense mediated decay | - | A0A2R8Y467 | - | - |
| BRAF-205 | ENST00000497784.2 | 2387 | 209aa | Nonsense mediated decay | - | H7C5K3 | - | TSL:5, |
| BRAF-213 | ENST00000644650.1 | 1639 | 411aa | Nonsense mediated decay | - | A0A2R8Y492 | - | CDS 5' incomplete, |
| BRAF-221 | ENST00000647434.1 | 1217 | 274aa | Nonsense mediated decay | - | A0A2R8Y679 | - | CDS 5' incomplete, |
| BRAF-203 | ENST00000479537.6 | 1129 | 231aa | Nonsense mediated decay | - | H7C4S5 | - | CDS 5' incomplete, TSL:5, |
| BRAF-212 | ENST00000644120.1 | 3889 | No protein | Processed transcript | - | - | - | - |
| BRAF-211 | ENST00000643790.1 | 575 | No protein | Processed transcript | - | - | - | - |
| BRAF-208 | ENST00000642808.1 | 439 | No protein | Processed transcript | - | - | - | - |
| BRAF-214 | ENST00000644905.1 | 5217 | No protein | Retained intron | - | - | - | - |
| BRAF-209 | ENST00000642875.1 | 2871 | No protein | Retained intron | - | - | - | - |
| BRAF-207 | ENST00000642272.1 | 2320 | No protein | Retained intron | - | - | - | - |
| BRAF-218 | ENST00000646427.1 | 2265 | No protein | Retained intron | - | - | - | - |
| BRAF-216 | ENST00000645443.1 | 2190 | No protein | Retained intron | - | - | - | - |
| BRAF-217 | ENST00000646334.1 | 1779 | No protein | Retained intron | - | - | - | - |
| BRAF-210 | ENST00000643356.1 | 853 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BRAF |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BRAF had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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