CRISPR-U™ Gene Knockout Cell Line Strategy

BRAF Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BRAF Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BRAF
Gene id 673
Organism Homo sapiens
Official full symbol B-Raf proto-oncogene, serine/threonine kinase
Gene type protein-coding
Also known as B-RAF1, B-raf, BRAF1, NS7, RAFB1
Summary This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
Genomic regions Chromosome 7
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BRAF-204 ENST00000496384.7 9578 767aa Protein coding CCDS87555 H7C560 - TSL:5, GENCODE basic, APPRIS P3,
BRAF-220 ENST00000646891.1 6458 766aa Protein coding CCDS5863 P15056 - GENCODE basic, APPRIS ALT2,
BRAF-215 ENST00000644969.2 9807 807aa Protein coding - A0A2R8Y8E0 NM_001374258.1 GENCODE basic, APPRIS ALT2, MANE Select v0.92,
BRAF-201 ENST00000288602.11 2561 806aa Protein coding - A0A2U3TZI2 - TSL:1, GENCODE basic, APPRIS ALT2,
BRAF-202 ENST00000469930.2 1180 172aa Protein coding - A0A2R8YES9 - TSL:2, GENCODE basic,
BRAF-219 ENST00000646730.1 2829 584aa Nonsense mediated decay - A0A2R8YDP5 - -
BRAF-206 ENST00000642228.1 2574 300aa Nonsense mediated decay - A0A2R8Y467 - -
BRAF-205 ENST00000497784.2 2387 209aa Nonsense mediated decay - H7C5K3 - TSL:5,
BRAF-213 ENST00000644650.1 1639 411aa Nonsense mediated decay - A0A2R8Y492 - CDS 5' incomplete,
BRAF-221 ENST00000647434.1 1217 274aa Nonsense mediated decay - A0A2R8Y679 - CDS 5' incomplete,
BRAF-203 ENST00000479537.6 1129 231aa Nonsense mediated decay - H7C4S5 - CDS 5' incomplete, TSL:5,
BRAF-212 ENST00000644120.1 3889 No protein Processed transcript - - - -
BRAF-211 ENST00000643790.1 575 No protein Processed transcript - - - -
BRAF-208 ENST00000642808.1 439 No protein Processed transcript - - - -
BRAF-214 ENST00000644905.1 5217 No protein Retained intron - - - -
BRAF-209 ENST00000642875.1 2871 No protein Retained intron - - - -
BRAF-207 ENST00000642272.1 2320 No protein Retained intron - - - -
BRAF-218 ENST00000646427.1 2265 No protein Retained intron - - - -
BRAF-216 ENST00000645443.1 2190 No protein Retained intron - - - -
BRAF-217 ENST00000646334.1 1779 No protein Retained intron - - - -
BRAF-210 ENST00000643356.1 853 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BRAF
This KO Strategy loading
Red Cotton™ Notes Gene BRAF had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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