CRISPR-U™ Gene Knockout Cell Line Strategy

SOD2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SOD2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SOD2
Gene id 6648
Organism Homo sapiens
Official full symbol superoxide dismutase 2
Gene type protein-coding
Also known as GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD
Summary This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
Genomic regions Chromosome 6
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SOD2-210 ENST00000538183.7 14167 222aa Protein coding CCDS5265 P04179-1 NM_000636.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SOD2-214 ENST00000546087.5 2558 176aa Protein coding CCDS83141 - - TSL:2, GENCODE basic,
SOD2-206 ENST00000452684.2 2045 140aa Protein coding CCDS83142 G5E9P6 - TSL:1, GENCODE basic,
SOD2-201 ENST00000337404.8 991 183aa Protein coding CCDS34564 P04179-2 - TSL:2, GENCODE basic,
SOD2-203 ENST00000367055.8 990 222aa Protein coding CCDS5265 P04179-1 - TSL:1, GENCODE basic, APPRIS P1,
SOD2-205 ENST00000444946.6 917 162aa Protein coding CCDS83143 P04179-3 - TSL:2, GENCODE basic,
SOD2-202 ENST00000367054.6 815 183aa Protein coding CCDS34564 P04179-2 - TSL:2, GENCODE basic,
SOD2-213 ENST00000545162.5 571 190aa Protein coding - F5GYZ5 - CDS 3' incomplete, TSL:3,
SOD2-208 ENST00000535561.5 551 183aa Protein coding - F5H4R2 - CDS 3' incomplete, TSL:3,
SOD2-204 ENST00000401980.3 523 61aa Protein coding - G8JLJ2 - CDS 3' incomplete, TSL:4,
SOD2-209 ENST00000537657.5 507 111aa Protein coding - F5H3C5 - CDS 3' incomplete, TSL:2,
SOD2-215 ENST00000546260.5 4302 141aa Nonsense mediated decay - F5GXZ9 - TSL:5,
SOD2-212 ENST00000541573.5 573 No protein Processed transcript - - - TSL:4,
SOD2-211 ENST00000540491.1 454 No protein Processed transcript - - - TSL:3,
SOD2-207 ENST00000535459.5 581 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SOD2
This KO Strategy loading
Red Cotton™ Notes Gene SOD2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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