CRISPR-U™ Gene Knockout Cell Line
Strategy
SOD2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SOD2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SOD2 |
| Gene id | 6648 |
| Organism | Homo sapiens |
| Official full symbol | superoxide dismutase 2 |
| Gene type | protein-coding |
| Also known as | GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD |
| Summary | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SOD2-210 | ENST00000538183.7 | 14167 | 222aa | Protein coding | CCDS5265 | P04179-1 | NM_000636.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SOD2-214 | ENST00000546087.5 | 2558 | 176aa | Protein coding | CCDS83141 | - | - | TSL:2, GENCODE basic, |
| SOD2-206 | ENST00000452684.2 | 2045 | 140aa | Protein coding | CCDS83142 | G5E9P6 | - | TSL:1, GENCODE basic, |
| SOD2-201 | ENST00000337404.8 | 991 | 183aa | Protein coding | CCDS34564 | P04179-2 | - | TSL:2, GENCODE basic, |
| SOD2-203 | ENST00000367055.8 | 990 | 222aa | Protein coding | CCDS5265 | P04179-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| SOD2-205 | ENST00000444946.6 | 917 | 162aa | Protein coding | CCDS83143 | P04179-3 | - | TSL:2, GENCODE basic, |
| SOD2-202 | ENST00000367054.6 | 815 | 183aa | Protein coding | CCDS34564 | P04179-2 | - | TSL:2, GENCODE basic, |
| SOD2-213 | ENST00000545162.5 | 571 | 190aa | Protein coding | - | F5GYZ5 | - | CDS 3' incomplete, TSL:3, |
| SOD2-208 | ENST00000535561.5 | 551 | 183aa | Protein coding | - | F5H4R2 | - | CDS 3' incomplete, TSL:3, |
| SOD2-204 | ENST00000401980.3 | 523 | 61aa | Protein coding | - | G8JLJ2 | - | CDS 3' incomplete, TSL:4, |
| SOD2-209 | ENST00000537657.5 | 507 | 111aa | Protein coding | - | F5H3C5 | - | CDS 3' incomplete, TSL:2, |
| SOD2-215 | ENST00000546260.5 | 4302 | 141aa | Nonsense mediated decay | - | F5GXZ9 | - | TSL:5, |
| SOD2-212 | ENST00000541573.5 | 573 | No protein | Processed transcript | - | - | - | TSL:4, |
| SOD2-211 | ENST00000540491.1 | 454 | No protein | Processed transcript | - | - | - | TSL:3, |
| SOD2-207 | ENST00000535459.5 | 581 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SOD2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SOD2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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