CRISPR-U™ Gene Knockout Cell Line
Strategy
SNCG Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SNCG Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SNCG |
| Gene id | 6623 |
| Organism | Homo sapiens |
| Official full symbol | synuclein gamma |
| Gene type | protein-coding |
| Also known as | BCSG1, SR |
| Summary | This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SNCG-202 | ENST00000372017.4 | 756 | 127aa | Protein coding | CCDS7380 | O76070 Q6FHG5 | NM_003087.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SNCG-201 | ENST00000348795.8 | 684 | 126aa | Protein coding | CCDS81483 | F8W754 | - | TSL:2, GENCODE basic, |
| SNCG-204 | ENST00000483064.1 | 794 | No protein | Processed transcript | - | - | - | TSL:2, |
| SNCG-203 | ENST00000465679.5 | 641 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SNCG |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SNCG had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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