CRISPR-U™ Gene Knockout Cell Line
Strategy
SMARCE1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SMARCE1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | SMARCE1 |
| Gene id | 6605 |
| Organism | Homo sapiens |
| Official full symbol | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 |
| Gene type | protein-coding |
| Also known as | BAF57, CSS5 |
| Summary | The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 24 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| SMARCE1-202 | ENST00000348513.12 | 5150 | 411aa | Protein coding | CCDS11370 | Q969G3-1 | NM_003079.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| SMARCE1-226 | ENST00000643683.1 | 2492 | 411aa | Protein coding | CCDS11370 | Q969G3-1 | - | GENCODE basic, APPRIS P1, |
| SMARCE1-223 | ENST00000643318.1 | 5796 | 341aa | Protein coding | - | Q969G3-3 | - | GENCODE basic, |
| SMARCE1-201 | ENST00000264640.9 | 2997 | 363aa | Protein coding | - | Q969G3-2 | - | TSL:3, GENCODE basic, |
| SMARCE1-250 | ENST00000647508.1 | 2840 | 376aa | Protein coding | - | Q969G3-4 | - | GENCODE basic, |
| SMARCE1-227 | ENST00000643806.1 | 2828 | 51aa | Protein coding | - | A0A2R8YDD9 | - | GENCODE basic, |
| SMARCE1-251 | ENST00000647515.1 | 2811 | 277aa | Protein coding | - | A0A2R8Y765 | - | GENCODE basic, |
| SMARCE1-204 | ENST00000400122.8 | 2600 | 293aa | Protein coding | - | Q969G3-6 | - | TSL:1, GENCODE basic, |
| SMARCE1-232 | ENST00000644527.1 | 2502 | 335aa | Protein coding | - | A0A2R8Y4T4 | - | GENCODE basic, |
| SMARCE1-233 | ENST00000644701.1 | 2488 | 363aa | Protein coding | - | Q969G3-2 | - | GENCODE basic, |
| SMARCE1-220 | ENST00000642459.1 | 2376 | 297aa | Protein coding | - | A0A2R8Y7U4 | - | GENCODE basic, |
| SMARCE1-203 | ENST00000377808.9 | 2348 | 328aa | Protein coding | - | Q969G3-5 | - | TSL:1, GENCODE basic, |
| SMARCE1-216 | ENST00000580419.6 | 2283 | 311aa | Protein coding | - | A0A2U3TZQ7 | - | TSL:1, GENCODE basic, |
| SMARCE1-242 | ENST00000646283.1 | 2229 | 347aa | Protein coding | - | A0A2R8YES3 | - | GENCODE basic, |
| SMARCE1-208 | ENST00000474246.2 | 2156 | 77aa | Protein coding | - | J3QL66 | - | TSL:1, GENCODE basic, |
| SMARCE1-235 | ENST00000645104.1 | 2118 | 332aa | Protein coding | - | A0A2R8Y7I9 | - | GENCODE basic, |
| SMARCE1-206 | ENST00000447024.6 | 1973 | 363aa | Protein coding | - | Q969G3-2 | - | TSL:3, GENCODE basic, |
| SMARCE1-213 | ENST00000578044.6 | 1704 | 341aa | Protein coding | - | Q969G3-3 | - | TSL:1, GENCODE basic, |
| SMARCE1-205 | ENST00000431889.6 | 1569 | 393aa | Protein coding | - | B4DGM3 | - | TSL:2, GENCODE basic, |
| SMARCE1-245 | ENST00000646482.1 | 1320 | 367aa | Protein coding | - | A0A2R8Y855 | - | GENCODE basic, |
| SMARCE1-209 | ENST00000478349.7 | 1086 | 287aa | Protein coding | - | J3QKS7 | - | CDS 3' incomplete, TSL:5, |
| SMARCE1-217 | ENST00000580654.6 | 888 | 259aa | Protein coding | - | J3QR61 | - | CDS 3' incomplete, TSL:5, |
| SMARCE1-218 | ENST00000582955.6 | 559 | 135aa | Protein coding | - | J3QKX6 | - | CDS 3' incomplete, TSL:5, |
| SMARCE1-212 | ENST00000577721.6 | 488 | 109aa | Protein coding | - | J3KT85 | - | CDS 3' incomplete, TSL:5, |
| SMARCE1-248 | ENST00000647294.1 | 2491 | 55aa | Nonsense mediated decay | - | A0A2R8YDQ4 | - | - |
| SMARCE1-214 | ENST00000578112.6 | 2388 | 52aa | Nonsense mediated decay | - | J3QS32 | - | TSL:5, |
| SMARCE1-238 | ENST00000645227.1 | 2298 | 58aa | Nonsense mediated decay | - | A0A2R8YFC8 | - | - |
| SMARCE1-246 | ENST00000646856.1 | 2026 | 52aa | Nonsense mediated decay | - | J3QS32 | - | - |
| SMARCE1-234 | ENST00000644909.1 | 1971 | 115aa | Nonsense mediated decay | - | A0A2R8YD78 | - | - |
| SMARCE1-210 | ENST00000481231.6 | 789 | 77aa | Nonsense mediated decay | - | J3QL66 | - | TSL:3, |
| SMARCE1-239 | ENST00000645478.1 | 584 | 96aa | Nonsense mediated decay | - | A0A2R8YEB8 | - | CDS 5' incomplete, |
| SMARCE1-231 | ENST00000644523.1 | 2305 | No protein | Processed transcript | - | - | - | - |
| SMARCE1-241 | ENST00000646242.1 | 8466 | No protein | Retained intron | - | - | - | - |
| SMARCE1-230 | ENST00000644443.1 | 4146 | No protein | Retained intron | - | - | - | - |
| SMARCE1-243 | ENST00000646401.1 | 3644 | No protein | Retained intron | - | - | - | - |
| SMARCE1-221 | ENST00000642576.1 | 3405 | No protein | Retained intron | - | - | - | - |
| SMARCE1-222 | ENST00000643030.1 | 2906 | No protein | Retained intron | - | - | - | - |
| SMARCE1-207 | ENST00000469334.6 | 2856 | No protein | Retained intron | - | - | - | TSL:5, |
| SMARCE1-224 | ENST00000643378.1 | 2832 | No protein | Retained intron | - | - | - | - |
| SMARCE1-236 | ENST00000645152.1 | 2575 | No protein | Retained intron | - | - | - | - |
| SMARCE1-244 | ENST00000646448.1 | 2519 | No protein | Retained intron | - | - | - | - |
| SMARCE1-228 | ENST00000643893.1 | 2496 | No protein | Retained intron | - | - | - | - |
| SMARCE1-247 | ENST00000647221.1 | 2377 | No protein | Retained intron | - | - | - | - |
| SMARCE1-211 | ENST00000493660.6 | 2319 | No protein | Retained intron | - | - | - | TSL:3, |
| SMARCE1-229 | ENST00000644257.1 | 2271 | No protein | Retained intron | - | - | - | - |
| SMARCE1-225 | ENST00000643580.1 | 1631 | No protein | Retained intron | - | - | - | - |
| SMARCE1-240 | ENST00000645663.1 | 1096 | No protein | Retained intron | - | - | - | - |
| SMARCE1-237 | ENST00000645155.1 | 734 | No protein | Retained intron | - | - | - | - |
| SMARCE1-215 | ENST00000578995.6 | 733 | No protein | Retained intron | - | - | - | TSL:2, |
| SMARCE1-249 | ENST00000647347.1 | 581 | No protein | Retained intron | - | - | - | - |
| SMARCE1-219 | ENST00000583294.1 | 476 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | SMARCE1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene SMARCE1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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